The column with a 4.6mm ID may not be sufficient to obtain around 10 mg of product by LC-MS purification. The maximum mass load for this column size would typically be around 500ug.
1.50458
SeQuant® ZIC®-HILIC 200Å (5 µm) HPLC Columns
L × I.D. 250 mm x 4.6 mm HPLC Column, PEEK-lined stainless steel
Sinónimos:
Zwitterionic Hydrophilic Interaction Liquid Chromatography HPLC Column
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| Tamaño de envase | SKU | Disponibilidad | Precio |
|---|---|---|---|
| 1 unit | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 1200,00 € |
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UNSPSC Code:
41115709
NACRES:
NB.21
Particle size:
5 μm
Matrix active group:
ZIC-HILIC, ZIC-HILIC bonding phase
Pore size:
200 Å pore size
Matrix:
fully porous particle
1200,00 €
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
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Permítanos ayudarleNombre del producto
SeQuant® ZIC-HILIC™ 5µm,200Å 250 x 4.6 mm, PEEK-lined stainless steel column, suitable for HPLC
material
PEEK-lined stainless steel column
Quality Level
agency
suitable for USP L114
product line
SeQuant®
feature
endcapped: no
parameter
500 bar pressure, 70 °C max. temp.
technique(s)
HPLC: suitable, LC/MS: suitable, mass spectrometry (MS): suitable (High)
column L × I.D.
25 cm × 4.6 mm
matrix
fully porous particle
matrix active group
ZIC-HILIC, ZIC-HILIC bonding phase
particle size
5 μm
pore size
200 Å pore size
operating pH
2-7.5
separation technique
hydrophilic interaction (HILIC)
storage temp.
no temp limit
General description
SeQuant® ZIC®-HILIC Columns are designed to retain and separate all types of polar and hydrophilic compounds and for robust chromatography with high selectivity and reproducibility. The silica-based ZIC®-HILIC sorbent has a bonded stationary phase consisting of a highly polar, permanent zwitterion. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes the ZIC®-HILIC Column overall neutral, with weak, but important, ionic interactions. Tuning of the selectivity on the ZIC®-HILIC Column during method development is facilitated by the pH-inpendent permanent zwitterion, ensuring that only the analytes (and not the column) are affected during eluent optimization.The Pore size of 200 Å makes this column perfectly suitable for the separation of larger polar molecules under HILIC conditions.The column dimension of 250 mm length x 4.6 mm ID is the best choice for the separation of complex samples using a standard HPLC Instrument.Thanks to their favorable retention and robust performance, SeQuant® HILIC columns have become extremely popular in numerous laboratories around the world. For example, US FDA (United States Food and Drug Administration) has recommended SeQuant® ZIC®-HILIC for analysis of melamine and related substances. SeQuant® HILIC columns excel in stability and low bleed. These features make them particularly suitable for LC-MS applications where high background can lead to signal suppression and interference with quantitative measurements while also increasing instrument wear. Their high stability makes these high-performance HILIC columns also attractive for traditional HPLC applications.Learn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection Guide Brochure: A Practical Guide to HPLC - Method Development Guide
Analysis Note
Theoretical Plates (N/m) (Cytosine): ≥ 60000
Symmetry (Cytosine): 0.95 - 1.40
Symmetry (Cytosine): 0.95 - 1.40
Legal Information
SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC-HILIC is a trademark of Merck KGaA, Darmstadt, Germany
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Este artículo | |||
|---|---|---|---|
| separation technique hydrophilic interaction (HILIC) | separation technique hydrophilic interaction (HILIC) | separation technique hydrophilic interaction (HILIC) | separation technique hydrophilic interaction (HILIC) |
| particle size 5 μm | particle size 5 μm | particle size 5 μm | particle size 5 μm |
| matrix active group ZIC-HILIC bonding phase | matrix active group ZIC-HILIC bonding phase | matrix active group ZIC-HILIC bonding phase | matrix active group ZIC-HILIC bonding phase |
| pore size 200 Å pore size | pore size 200 Å pore size | pore size 200 Å pore size | pore size 200 Å pore size |
| agency suitable for USP L114 | agency suitable for USP L114 | agency suitable for USP L114 | agency suitable for USP L114 |
| technique(s) HPLC: suitable, mass spectrometry (MS): suitable (High), LC/MS: suitable | technique(s) HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable, mass spectrometry (MS): suitable (High) | technique(s) HPLC: suitable, LC/MS: suitable (Prep LC), mass spectrometry (MS): suitable (High) | technique(s) HPLC: suitable, LC/MS: suitable (Capillary LC), mass spectrometry (MS): suitable (High) |
Clase de almacenamiento
11 - Combustible Solids
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Número de artículo de comercio global
| SKU | GTIN |
|---|---|
| 1504580001 | 04027269134402 |
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Can a column with a 4.6mm ID be used to obtain around 10 mg of product by LC-MS purification? Also, what is the maximum mass load for your column with a 4.6mm ID?
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