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1.50247

Purospher® STAR NH2 (5 µm) HPLC Columns

L × I.D. 150 mm x 4.6 mm LiChroCART HPLC cartridge, manuCART holder required for use

Sinónimos:

Purospher® STAR NH2 (5 µm) LiChroCART 150-4.6

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1 unit
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764,00 €

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UNSPSC Code:
41115709
NACRES:
SB.52
Particle size:
5 μm
Matrix active group:
NH2, NH2 bonding phase
Pore size:
120 Å pore size
Matrix:
fully porous particle

764,00 €


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Nombre del producto

Purospher® STAR NH2 (5 µm), L × I.D. 150 mm x 4.6 mm LiChroCART HPLC cartridge, manuCART holder required for use

material

stainless steel column

Quality Segment

agency

suitable for USP L8

product line

Purospher®

feature

endcapped: no

packaging

1 ea of

extent of labeling

3.5% Carbon loading

parameter

250 bar pressure, 60 °C max. temp.

technique(s)

HPLC: suitable, LC/MS: suitable, mass spectrometry (MS): suitable (Low - Medium)

column L × I.D.

15 cm × 4.6 mm

surface area

330 m2/g

matrix

fully porous particle

matrix active group

NH2, NH2 bonding phase

particle size

5 μm

pore size

120 Å pore size

operating pH range

2-7.5

separation technique

hydrophilic interaction (HILIC), normal phase, reversed phase

storage temp.

no temp limit

General description

Purospher® STAR NH2, 5 µm HPLC columns are widely used for carbohydrate analysis, with a typical mobile phaseconsisting of acetonitrile and water. In terms of their polarity, these columns lie between bare silica and reversed-phase silica. Hence, Purospher® STAR NH2 can also be used as an ion-exchanger. In acidic solutions, the NH2-groups are protonated (-NH3 +X-) and therefore display the characteristics of a weak anion exchanger. Medium polarity Purospher® STAR NH2 columns possess hydrophilic as well as hydrophobic properties, thus can be used under both reversed-phase (HILIC) and normal-phase conditions. However, retention is weaker than on silica or RP-supports. Purospher® STAR NH2 columns are based on ultra-pure fully porous silica particles (Type B). Purospher® STAR NH2, 5 µm LiChroCART 150-4.6 is a cartridge column which requires a manuCART cartridge column holder part number 1.51486.0001.The recommended guard column is Purospher® STAR NH2 (5 µm) LiChroCART 4-4 part number 1.50267.0001. LiChroCART cartridge columns mounted with a manuCART cartridge column holder is hand-tight only and have therefore a maximum pressure stability of 250 bar.The column dimension of 150 mm length x 4.6 mm I.D. is the optimal choice for the HPLC separation of complex.Learn more about Purospher® STAR HPLC and UHPLC ColumnsLearn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection GuideBrochure: A Practical Guide to HPLC - Method Development Guide

Application

Intended to be used in the separation of alcohols,carboxylic acids, fat soluble vitamins, nucleotides, steroids, sugars, sugar alcohols, sweeteners, and water soluble vitamins.

Features and Benefits

  • Ultra-pure silica (99.999%)
  • Low silanol activity
  • Reproducible results from run-to-run and batch-to-batch

Analysis Note

Pressure: ≤ 45 bar
Theoretical Plates (N/m) (2-Nitrotoluene): ≥ 50000
Symmetry (2-Nitrotoluene): 0.8 - 1.4
Capacity factor (2-Nitrotoluene): ≤ 3.0

Legal Information

LICHROCART is a trademark of Merck KGaA, Darmstadt, Germany
MANUCART is a trademark of Merck KGaA, Darmstadt, Germany
Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany

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Este artículo
1.502451.502441.50248
matrix active group

NH2 bonding phase

matrix active group

NH2 bonding phase

matrix active group

NH2 bonding phase

matrix active group

NH2 bonding phase

separation technique

hydrophilic interaction (HILIC), reversed phase, normal phase

separation technique

hydrophilic interaction (HILIC), normal phase, reversed phase

separation technique

hydrophilic interaction (HILIC), normal phase, reversed phase

separation technique

hydrophilic interaction (HILIC), normal phase, reversed phase

particle size

5 μm

particle size

5 μm

particle size

5 μm

particle size

5 μm

pore size

120 Å pore size

pore size

120 Å pore size

pore size

120 Å pore size

pore size

120 Å pore size

product line

Purospher®

product line

Purospher®

product line

Purospher®

product line

Purospher®

matrix

fully porous particle

matrix

fully porous particle

matrix

fully porous particle

matrix

fully porous particle


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Preguntas

  1. Is the product Purospher® STAR NH2 (5 µm) LiChroCART® 150-4.6 column (catalog number 1.50247.0001 or 48219-474) compatible with the Agilent 1260 HPLC system?

    1 respuesta
    1. While most HPLC columns feature 10.32 threaded end fittings, some may have different types and sizes of fittings. Given the wide variety of HPLC systems, it is not possible to guarantee with complete certainty that a specific HPLC system utilizes 10.32 fittings. Furthermore, even within a single type of fitting, such as 10.32, there are short, standard, and extended versions of the nut and ferrule. Hence, it is advisable to consult the instrument manual to determine the correct fitting type and size.

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