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12-309

HeLa Nuclear Extract

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A ustedes/SKUDisponibilidadPrecio
50 μg
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
189,00 €

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UNSPSC Code:
41105330
NACRES:
NA.41
eCl@ss:
32011807
Biological source:
human
Manufacturer/tradename:
Upstate®

189,00 €

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biological source

human

Quality Segment

100

form

liquid

manufacturer/tradename

Upstate®

concentration

2.0 mg/mL

technique(s)

western blot: suitable

shipped in

dry ice

General description

Nuclear extract prepared from human HeLa cells using a modified protocol of Dignam, et al., which contains a variety of DNA binding proteins and transcription factors.

Biochem/physiol Actions

HeLa

Physical form

RIPA sample buffer containing inhibitors. Frozen solution.

Preparation Note

Stable for 6 months at -70°C from date of shipment.

Analysis Note

Routinely evaluated as a positive control for many of Upstates antibodies.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Este artículo
126-2.5NXTRACT171204C
HeLa Nuclear Extract

Sigma-Aldrich

12-309

HeLa Nuclear Extract

SAFC

SAFC

126-2.5

EHS Matrix Extract

NuCLEAR™ Extraction Kit For mammalian tissue or cultured cells

Sigma-Aldrich

NXTRACT

NuCLEAR™ Extraction Kit

Heart Extract Polar Avanti Research™ - A Croda Brand

Avanti

171204C

Heart Extract Polar

technique(s)

western blot: suitable

technique(s)

-

technique(s)

protein extraction: suitable, western blot: suitable

technique(s)

-

biological source

human

biological source

-

biological source

-

biological source

-

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

manufacturer/tradename

Upstate®

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

Avanti Research™ - A Croda Brand

Quality Level

100

Quality Level

100

Quality Level

300

Quality Level

-

form

liquid

form

liquid

form

-

form

chloroform solution

pictograms

Exclamation mark
GHS07

Clase de almacenamiento

12 - Non Combustible Liquids

wgk

WGK 3

signalword

Danger

Hazard Classifications

ED ENV 1 - Eye Irrit. 2

Listados normativos

Los listados normativos se proporcionan para los productos químicos principalmente. Para los productos no químicos sólo se puede proporcionar información limitada. Si no hay ninguna entrada, significa que ninguno de los componentes está en la lista. Es obligación del usuario garantizar el uso seguro y legal del producto.

127087-87-0

CAS No.

127087-87-0

CAS No.

127087-87-0

CAS No.

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Preguntas

1–2 de 2 Preguntas  

  1. Is this product in vitro transcription grade? What are the components provided with this item? Can I used this item to test for human rna polymerase 2 transcription?

    1 respuesta

    1. This product is not tested for a specific application, such as transcription efficiency. The material is supplied as a single vial of a 50 ug/25 uL solution containing RIPA buffer and inhibitors. No other components are included. Note that unless otherwise stated this product is intended for research use only and is not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

      Please see the link below to review available Certificates of Analysis:
      https://www.emdmillipore.com/product/HeLa-Nuclear-Extract,MM_NF-12-309#anchor_COA

      ¿Le ha resultado útil?

  2. Can I use this HeLa Nuclear Extract for deadenylase assay? specifically, Does this product contain the complex of deadenylase in addition to a variety of DNA binding proteins and transcription factors?

    1 respuesta

    1. This product has not been tested for use in deadenylation. There is no reason to suspect that it would not be suitable. However, the product is stored in RIPA buffer. Please check the literature for potential interference of RIPA in the adenylase assay reaction.

      ¿Le ha resultado útil?

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