Product contains ampoules with spores of Bacillus subtilius, no vegetative cells. The expiration date of the product is on the label of the product box; the shelf life of the product is 17 months when stored at the given storage temperature.
The spores can directly be added to the melted agar, with an agar temperature of not more than 50°C. Please consult the technical data sheet of the product: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/299/903/110649-bacillus-subtilis-bga-spore-suspension-for-the-inhibitor-test-ms.pdf


