The gelation process for this product does not require pH neutralization by the addition of acid or base. The procedure involves pipetting undiluted collagen into culture dishes, exposing them to ammonia vapor, and rinsing with sterile distilled water multiple times to remove ammonia. The final rinse is replaced with a sterile medium or balanced salt solution with serum, and the dishes are stored at 4°C until use.
08-115
Rat Collagen Type I
from rat tail, liquid, 2-5 mg/mL, suitable for cell culture, used for 3D gel formation
Sinónimos:
Colágeno de cola de rata, Colágeno de cola de rata tipo I
Iniciar sesión para ver los precios por organización y contrato.
Seleccione un Tamaño
Cambiar Vistas
| Tamaño de envase | SKU | Disponibilidad | Precio |
|---|---|---|---|
| 100 mg | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 370,00 € |
Acerca de este artículo
UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.75
Biological source:
rat
Form:
liquid, solution
Technique(s):
cell culture | mammalian: suitable (and for 3D proliferation assays.)
Concentration:
100 mg
370,00 €
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
Servicio técnico
¿Necesita ayuda? Nuestro equipo de científicos experimentados está aquí para ayudarle.
Permítanos ayudarleNombre del producto
Colágeno tipo I, cola de rata, 100 mg, solution, BioReagent, suitable for cell culture
biological source
rat
Quality Level
form
liquid, solution
manufacturer/tradename
Upstate®
concentration
100 mg
technique(s)
cell culture | mammalian: suitable (and for 3D proliferation assays.)
input
sample type hematopoietic stem cell(s)
sample type mesenchymal stem cell(s)
sample type induced pluripotent stem cell(s)
sample type epithelial cells
sample type pancreatic stem cell(s)
sample type neural stem cell(s)
sample type: mouse embryonic stem cell(s)
NCBI accession no.
UniProt accession no.
shipped in
wet ice
storage temp.
2-8°C
Gene Information
rat ... COL1A1(29393)
General description
En los seres humanos, el colágeno tipo I es el colágeno más abundante y ampliamente expresado. Este heterotrímero contiene dos cadenas alfa 1 (I) y una cadena alfa 2 (I).[1] El colágeno tipo I α 1 (COL1A1), un componente importante del colágeno tipo I, codifica las cadenas de pro-α 1 del colágeno tipo I.[2] Tiene motivos de tripéptidos de Gly-Xaa-Yaa repetidos. La cadena proteica alfa-1(I) del colágeno es un miembro de la superfamilia del complejo del colágeno.[1] El COL1A1 tiene un dominio del factor von Willebrand tipo C (vWFC), una repetición de triple hélice del colágeno y un dominio C-terminal de colágeno fibrilar (COLF).[2]
Application
Se ha utilizado el colágeno tipo I de cola de rata:
- para ensayos de proliferación de colágeno en 3D[1]
- para ensayos de proliferación de atelocolágeno[1]
- en un estudio comparativo para hacer microcápsulas para plantillas en núcleos mesoporosos de cristales puros de carbonato de calcio (CaCO3) de vaterita para evaluar la relación estructura-propiedad[2]
- en un recubrimiento de cubreobjetos para hacer crecer la línea de células endoteliales microvasculares cerebrales humanas (hCMEC/D3) para crear un modelo de lesión inducida por muestra de hematoma de un paciente con cocultivo celular y hematoma subdural crónico (CSDH)[3]
Biochem/physiol Actions
El colágeno tipo I α 1 (COL1A1) desempeña un papel clave en la quimiorresistencia y puede actuar como diana terapéutica. Está involucrado en el cáncer de ovario (OV) resistente al carboplatino.[1] El COL1A1 actúa como oncogén en el desarrollo del cáncer colorrectal (CCR).[2] Las mutaciones hereditarias dominantes del gen COL1A1 provocan trastornos del tejido conjuntivo como la osteogénesis imperfecta (OI).[1]
Physical form
Ácido acético 0,02 N, pH 3,67
Preparation Note
1 año a 4°C
Analysis Note
Se evalúa sistemáticamente analizando la gelación uniforme y la fijación y propagación de células PC-12 de rata
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Salvo que se indique lo contrario en nuestro catálogo o en otra documentación de la empresa que acompañe al producto, nuestros productos están indicados sólo para investigación y no deben utilizarse para ningún otro propósito, como, entre otros, usos comerciales no autorizados, diagnóstico in vitro, usos terapéuticos ex vivo o in vivo o cualquier tipo de consumo o aplicación en humanos o animales.
1 of 1
Este artículo | |||
|---|---|---|---|
| biological source rat | biological source rat tail | biological source human skin | biological source rabbit |
| technique(s) cell culture | mammalian: suitable (and for 3D proliferation assays.) | technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) ELISA: suitable, immunohistochemistry: suitable, radioimmunoassay: suitable, western blot: suitable |
| concentration 100 mg | concentration - | concentration - | concentration - |
| Quality Level 100 | Quality Level - | Quality Level 100 | Quality Level 100 |
| form liquid, solution | form lyophilized (clear, colorless solution after reconstitution) | form lyophilized | form - |
| Gene Information rat ... COL1A1(29393) | Gene Information rat ... Col1a1(29393) | Gene Information - | Gene Information human ... COL1A1(1277) |
Still not finding the right product?
Explore all of our products under Colágeno tipo I, cola de rata
Clase de almacenamiento
12 - Non Combustible Liquids
wgk
WGK 1
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Protocolos
3D cell culture protocol for generating epidermal human skin tissue using primary human keratinocytes, dermal fibroblasts, and collagen-coated transwell inserts.
Artículos
The extracellular matrix (ECM) and its attachment factor components are discussed in this article in relation to their function in structural biology and their availability for in vitro applications.
Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.
Contenido relacionado
This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.
Kerim B Kaylan et al.
Scientific reports, 6, 23490-23490 (2016-03-31)
The bipotential differentiation of liver progenitor cells underlies liver development and bile duct formation as well as liver regeneration and disease. TGFβ and Notch signaling are known to play important roles in the liver progenitor specification process and tissue morphogenesis.
M Yebra et al.
The Journal of biological chemistry, 271(46), 29393-29399 (1996-11-15)
The urokinase plasminogen activator (uPA) interacts with its cell surface receptor (uPAR), providing an inducible, localized cell surface proteolytic activity, thereby promoting cellular invasion. Evidence is provided for a novel function of cell surface-associated uPA.uPAR. Specifically, induction of cell surface
C Fink et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 14(5), 669-679 (2000-04-01)
To examine the influence of chronic mechanical stretch on functional behavior of cardiac myocytes, we reconstituted embryonic chick or neonatal rat cardiac myocytes to a 3-dimensional engineered heart tissue (EHT) by mixing freshly isolated cells with neutralized collagen I and
Número de artículo de comercio global
| SKU | GTIN |
|---|---|
| 08-115 | 08436037123627 |
-
Does this product require pH neutralization by addition of acid or base for gelation?
1 respuesta-
¿Le ha resultado útil?
-
-
Hi team: I hope this finds you well. May I know the concentration of collagen type I , rat tail 08-115? Much appreciated. Kind regards, Ovi
1 respuesta-
The concentration is provided in lot specific Certificates of Analysis, typically within the range of 3 to 5 mg/ml.
¿Le ha resultado útil?
-
-
Accidentally, I opened the collagen out of the flow chamber. Should I filter it or autoclave it to ensure the sterile conditions?
1 respuesta-
Collagen solutions may be sterilized by dialysis in a 0.25% acetic acid and 0.5% chloroform solution.
Avoid sterilizing the collagen solution by membrane filtration, as substantial protein loss may occur by this method. The coating of tissue culture plastic dishes may be performed by air-drying. Dried coated dishes may then be sterilized by exposure to UV light in a sterile culture hood or by rinsing with 70% ethanol.¿Le ha resultado útil?
-
-
Hi, what is the concentration of the collagen?
1 respuesta-
The concentration of this product will be listed on the lot-specific Certificate of Analysis. Please see the link below to access a Certificate:
https://www.emdmillipore.com/US/en/product/Collagen-Type-I-rat-tail,MM_NF-08-115#anchor_COA¿Le ha resultado útil?
-
-
Once diluted in 0.02N acetic acid, does the collagen solution require sterilisation for use in cell culture? If so, which method is the most effective: UV, autoclave or 0.22uM sterile filter (should this be PES or PVDF)?
1 respuesta-
This product is supplied as a sterile solution in 0.02 N Acetic acid. The package size is 100 mg, however, the concentration is variable and reported on the lot-specific Certificate of Analysis. The typical range is 4 - 5 mg/mL. Dilutions can be made with sterile PBS. Further sterilization is not required. If necessary, coated slides and coverslips can be sterilized by UV.
¿Le ha resultado útil?
-