L1754

Lipase from Candida rugosa

Type VII, ≥700 unit/mg solid

• Szinonimák: Triacylglycerol acylhydrolase, Triacylglycerol lipase
• CAS-szám: 9001-62-1
• Enzyme Commission szám: 3.1.1.3 (BRENDA, IUBMB)
• EC-szám: 232-619-9
• MDL-szám: MFCD00131509
• UNSPSC kód: 12352204
• NACRES: NA.54

Tulajdonságok

• biológiai forrás: fungus (candida rugosa)
• Minőségi szint: 200
• típus: Type VII
• form: lyophilized
• specifikus aktivitás: ≥700 unit/mg solid
• tárolási körülmény: (Tightly closed. Dry)
• technika/technikák: cell based assay: suitable
• szín: beige; white
• oldhatóság: water: slightly soluble
• tárolási hőmérséklet: 2-8°C
• InChI: 1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
• Nemzetközi kémiai azonosító kulcs: QWZUIMCIEOCSJF-CHHCPSLASA-N

Leírás

Általános leírás

Research area: Cell Structure

Lipases are found in pancreatic secretions. This class of enzymes contains α and β hydrolase folds. Candida rugosa produces multiple lipase isoenzymes that have 80% sequence homology.

This product contains lactose as a carrier.

Alkalmazás

Lipase from Candida rugosa has been used:

• to synthesize dextran fatty acid esters
• to study the effect of lipase on conjugated linoleic acid (CLA1 and CLA2) production in the presence of sunflower oil and castor oil
• to prepare pH-imprinted enzyme for lipase-catalyzed transesterification of dextran T-40 with vinyl decanoate

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Biokémiai/fiziológiai hatások

Lipases are enzymes and biocatalysts that catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids. They are useful for biotransformation reactions. It possesses broad substrate tolerance and is highly stereo- and regioselective. Lipases mediate lipid transport. Elevated levels of serum lipase are observed in pancreatitis. Lipases are the preferred catalysts for long-chain fatty acids. Lipases can be used in a wide range of solvents, in both aqueous and non-aqueous media, and thus have found use in applications like organic synthesis.

Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).

Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.

Egység definíció

One unit will hydrolyze 1.0 microequivalent of fatty acid from a triglyceride in 1 hr at pH 7.2 at 37 °C. (This is equivalent to approx. 10 microliters of CO₂ in 30 minutes.)

Biztonsági adatok

• Piktogramok: GHS08
• Figyelmeztetés: Danger
• Figyelmeztető mondatok: H334
• Óvintézkedésre vonatkozó mondatok: P261 - P284 - P501
• Veszélyességi osztályok: Resp. Sens. 1
• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves, type N95 (US)