L0382

Lipase from porcine pancreas

Type VI-S, ≥20,000 units/mg protein, lyophilized powder

• Szinonimák: PPL, Triacylglycerol acylhydrolase, Triacylglycerol lipase
• CAS-szám: 9001-62-1
• Enzyme Commission szám: 3.1.1.3 (BRENDA, IUBMB)
• EC-szám: 232-619-9
• MDL-szám: MFCD00131509
• UNSPSC kód: 12352204
• NACRES: NA.54

Tulajdonságok

• típus: Type VI-S
• Minőségi szint: 300
• form: lyophilized powder
• specifikus aktivitás: ≥20,000 units/mg protein
• összetétel: Protein, 40-70%
• kiszállítva: wet ice
• tárolási hőmérséklet: −20°C
• InChI: 1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
• Nemzetközi kémiai azonosító kulcs: QWZUIMCIEOCSJF-CHHCPSLASA-N

Leírás

Általános leírás

Lipase from porcine pancreas (PPL) or porcine pancreatic lipase is a small globular protein with N-terminal α/β type fold. It comprises catalytic triad Ser, Asp, and His residues and a C-terminal β-sandwich domain. PPL belongs to a subclass of the carboxylesterases family.

Alkalmazás

Lipase from porcine pancreas has been used:

• in lipase inhibition assay with plant extracts
• in in vitro digestion gelatinized starch/fiber mixtures
• with simulated intestinal fluid to mimic the digestive tract condition of by L. fermentum PC1 in fermented oats

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Biokémiai/fiziológiai hatások

Lipase from porcine pancreas (PPL) catalyzes the hydrolysis of fats (lipids). It plays an essential role in the digestion, transport, and processing of dietary lipids (e.g. triglycerides, fats, oils) in most living organisms. PPL is cost-effective and is useful in biotransformation reactions. It displays high selectivity, and catalytic activity. PPL is also used in the detergent formulation for removing oil stains from clothes.

Lipases catalyze the hydrolysis of esters in aqueous solutions and the synthesis of esters in non-aqueous solutions. They also produce hydroxyl and carboxylic groups through the hydrolysis of ester linkages in poly(ethylene terephthalate).

Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.

Egység definíció

One unit will hydrolyze 1.0 microequivalent of fatty acid from a triglyceride in 1 hr at pH 7.7 at 37 °C using olive oil.

Analízis megjegyzés

Protein determined by biuret.

Biztonsági adatok

• Piktogramok: GHS08
• Figyelmeztetés: Danger
• Figyelmeztető mondatok: H334
• Óvintézkedésre vonatkozó mondatok: P261 - P284 - P501
• Veszélyességi osztályok: Resp. Sens. 1
• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves, type N95 (US)