AP112B
Goat Anti-Human IgG Antibody, biotin conjugate
Chemicon®, from goat
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.46
Productos recomendados
origen biológico
goat
Nivel de calidad
conjugado
biotin conjugate
forma del anticuerpo
affinity purified immunoglobulin
tipo de anticuerpo
secondary antibodies
clon
polyclonal
reactividad de especies
human
fabricante / nombre comercial
Chemicon®
técnicas
ELISA: suitable
western blot: suitable
Condiciones de envío
wet ice
modificación del objetivo postraduccional
unmodified
Descripción general
IgG is a monomeric immunoglobulin, consists of two heavy chains (gamma chains) and two light chains. Each molecule has two antigen binding sites. IgG is the most abundant immunoglobulin. It is approximately equally distributed in blood and in interstitial fluid, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, providing protection to the fetus in its first weeks of life before its own immune system has developed. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).
Especificidad
The antibody reacts with the heavy chains of human IgG and with the light chains common to most human immunoglobulins.
Inmunógeno
Prepared from purified human IgG.
Aplicación
EIA: 1:20,000-1:400,000 using enzyme-streptavidin conjugate.
Western blots: 1:20,000-1:400,000 using enzyme-streptavidin conjugate.
Immunohistochemistry: 1:500-1:5,000 using enzyme-streptavidin conjugate.
Flow cytometry: 1:200-1:1,000.
Fluorescence Immunohisto/cytochemistry: 1:200-1:1,1000.
Optimal working dilutions must be determined by the end user.
Western blots: 1:20,000-1:400,000 using enzyme-streptavidin conjugate.
Immunohistochemistry: 1:500-1:5,000 using enzyme-streptavidin conjugate.
Flow cytometry: 1:200-1:1,000.
Fluorescence Immunohisto/cytochemistry: 1:200-1:1,1000.
Optimal working dilutions must be determined by the end user.
Research Category
Secondary & Control Antibodies
Secondary & Control Antibodies
Research Sub Category
Whole Immunoglobulin Secondary Antibodies
Whole Immunoglobulin Secondary Antibodies
This Goat anti-Human IgG Antibody, biotin conjugate is validated for use in ELISA, WB for the detection of Human IgG.
Forma física
ImmunoAffinity Purified
Purified by immunoaffinity chromatography using antigen coupled to agarose beads. Lyophilized. Buffer is 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA, and 0.05% sodium azide.
Almacenamiento y estabilidad
Maintain lyophilized product at 2°-8°C for up to 12 months from date of receipt. After reconstitution the product is stable for six weeks at 2°-8°C as an undiluted liquid. Reconstitute vial with 2 mL of distilled water. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of antibody (and buffer salts) will decrease to one-half of the original after the addition of glycerol and final working dilutions must be adjusted accordingly. Avoid repeated freeze thaw cycles.
Información legal
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 3
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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