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C9658

Carboxypeptidase G from Pseudomonas sp.

Name: Carboxypeptidase G from <I>Pseudomonas</I> sp.
Brand: SIGMA
Price: 5277 MXP

lyophilized powder, ≥3 units/mg protein

Sinónimos:

γ-Glutamyl hydrolase

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Tamaño de envase	SKU	Disponibilidad	Precio
5 units		Comprobar disponibilidad del carrito	MXP 5,277.00
20 units		Comprobar disponibilidad del carrito	MXP 18,161.00 MXP 15,436.85

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Número CAS:

9074-87-7

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-978-1

MDL number:

MFCD00130719

Número CE:

3.4.17.11 (BRENDA, IUBMB)

Specific activity:

≥3 units/mg protein

MXP 5,277.00

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Propiedades

form

lyophilized powder

Quality Segment

300

specific activity

≥3 units/mg protein

mol wt

homodimer ~90 kDa

composition

Protein, ~70% biuret

solubility

H₂O: soluble 0.5 mg/mL

storage temp.

−20°C

Descripción

Application

Carboxypeptidase G from Pseudomonas sp., or γ-Glutamyl hydrolase, has been used in a study to assess the role of the putidaredoxin COOH-terminus in P-450cam (cytochrome m) hydroxylations. Carboxypeptidase G from Pseudomonas sp. has also been used in a study to investigate the effects of nitric oxide on pemetrexed cytotoxicity via NO‑cGMP signaling in lung adenocarcinoma cells.

Biochem/physiol Actions

Carboxypeptidase G is a lysosomal, thiol-dependent protease, which progressively cleaves γ-glutamyl pteroyl poly-γ-glutamate yielding pteroyl-α-glutamate (folic acid) and free glutamate. It is considered highly specific for the γ-glutamyl bond, but not for the C-terminal amino acid of the leaving group.[1] Molecular mass of this homodimer is approximately 90 kDa. The enzyme is activated by Zn²⁺ ions.

Physical form

Contains sodium acetate salt.

Preparation Note

Chromatographically purified

Solutions should be prepared fresh prior to use.

Other Notes

One unit will hydrolyze 1.0 μmole of L-glutamic acid from (+)amethopterin per min at pH 7.3 at 30 °C.

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Este artículo	C9584	C9268	C3888
Sigma-Aldrich C9658 Carboxypeptidase G from Pseudomonas sp.	Sigma-Aldrich C9584 Carboxypeptidase B from porcine pancreas	Sigma-Aldrich C9268 Carboxypeptidase A from bovine pancreas	Sigma-Aldrich C3888 Carboxypeptidase Y from baker′s yeast (S. cerevisiae)
specific activity ≥3 units/mg protein	specific activity ≥125 units/mg protein	specific activity ≥50 units/mg protein	specific activity ≥50 units/mg protein
form lyophilized powder	form lyophilized powder	form ready-to-use solution	form lyophilized powder
storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C	storage temp. −20°C
mol wt homodimer ~90 kDa	mol wt 34,000 Da± 600	mol wt ~35 kDa	mol wt 61 kDa
solubility H₂O: soluble 0.5 mg/mL	solubility -	solubility -	solubility -
Quality Level 300	Quality Level 200	Quality Level 300	Quality Level 200