The stability of this material following multiple freeze/thaw cycles indicates that a fine precipitate may be evident. However, the performance of the material was is effected. While it is recommended to use this product within 5 days of the initial thaw, it would be up to the end user to continue use following refreezing. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/241/461/c6295pis.pdf
C6295
Cell Freezing Medium-DMSO Serum free 1x
sterile-filtered, suitable for cell culture
Sinónimos:
cryopreservation medium
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| Tamaño de envase | SKU | Disponibilidad | Precio |
|---|---|---|---|
| 50 mL | Comprobar disponibilidad del carrito | MXP 2,859.00 |
Acerca de este artículo
NACRES:
NA.75
UNSPSC Code:
12352207
MXP 2,859.00
Comprobar disponibilidad del carrito
Servicio técnico
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Permítanos ayudarlesterility
sterile-filtered
Quality Segment
form
liquid
technique(s)
cell culture | mammalian: suitable, cryopreservation: suitable
impurities
endotoxin, tested
shipped in
dry ice
storage temp.
−20°C
General description
Cell freezing medium is a combination of cryoprotectant DMSO with fetal bovine serum. However serum free medium replaces serum with methylcellulose and poloxamer 188 and other additives like ascorbic acid, α-tocopherol and proline.
Application
Cell Freezing Medium-DMSO Serum free 1x has been used in the freezing of human neural progenitor cells (hNPCs), olfactory ensheathing cells (OECs) and retinal pigment epithelial (RPE) cells.
Other Notes
Complete ready-to-use medium containing Minimum Essential Medium, dimethyl sulfoxide (8.7%) and methyl cellulose. Prepared according to the formulation of Waymouth.
1 of 1
Este artículo | |||
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable, cryopreservation: suitable | technique(s) cell culture | mammalian: suitable, cryopreservation: suitable | technique(s) cell culture | mammalian: suitable, cryopreservation: suitable | technique(s) cell culture | mammalian: suitable |
| sterility sterile-filtered | sterility - | sterility sterile-filtered | sterility sterile-filtered |
| form liquid | form liquid | form liquid | form liquid |
| shipped in dry ice | shipped in wet ice | shipped in dry ice | shipped in dry ice |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
Clase de almacenamiento
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Contenido relacionado
Acute and delayed transplantation of olfactory ensheathing cells promote partial recovery after complete transection of the spinal cord
Lopez-Vales R, et al.
Neurobiology of Disease, 21(1), 57-68 (2006)
A novel serum-free method for culturing human prenatal retinal pigment epithelial cells
Gamm DM, et al.
Investigative Ophthalmology & Visual Science, 49(2), 788-799 (2008)
Glial cell line-derived neurotrophic factor-secreting human neural progenitors show long-term survival, maturation into astrocytes, and no tumor formation following transplantation into the spinal cord of immunocompromised rats.
Gowing G, et al.
Neuroreport, 25(6), 367-367 (2014)
Número de artículo de comercio global
| SKU | GTIN |
|---|---|
| C6295-50ML | 04061833511114 |
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Is it possible to thaw this product, prepare aliquots and store them at -20 degrees for further use? Thank you.
1 answer-
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