Anhidrasa carbónica from bovine erythrocytes

Name: Anhidrasa carbónica from bovine erythrocytes
Brand: SIGMA

For use as a marker in SDS-PAGE

Sinónimos:

Carbonato deshidratasa, Carbonato hidrolasa

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Número CAS:

9001-03-0

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-576-6

MDL number:

MFCD00081467

Número CE:

4.2.1.1 (BRENDA, IUBMB)

Biological source:

bovine erythrocytes

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Propiedades

biological source

bovine erythrocytes

Quality Segment

200

form

powder

mol wt

~29 kDa

packaging

vial of 5 mg

storage temp.

2-8°C

Descripción

General description

Carbonic Anhydrase is a metalloenzyme with zinc.[1] The active site of carbonic anhydrase contains three conserved histidine residues (His94, His96 and His119).[2]

Application

Carbonic Anhydrase has been used:

as a component of nine-protein mixture for the comparative proteomic mass spectrometry analysis of bovine brain samples[3]
as a molecular marker in gel filtration chromatography[4]
as a standard protein to test the recovery of CMA/20 microdialysis probe[5]

Biochem/physiol Actions

Carbonic anhydrase is essential for the reversible hydration of carbon dioxide.[1] It is also crucial for the physiological processes including respiration, ion transport, acid–base balance, lipid and carbohydrate metabolic pathways.[1] Carbonic anhydrase participates in electrolyte secretion and in the carbon dioxide fixation.[2]

Other Notes

One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C.