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B2787

Biotinylated Molecular Weight Marker

mol wt 6,500-180,000 Da

Sinónimos:

protein markers, protein molecular weight markers, protein standards

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Tamaño de envaseSKUDisponibilidadPrecio
1 vial
Comprobar disponibilidad del carrito
MXP 4,572.00
5 x 1 vial
Comprobar disponibilidad del carrito
MXP 18,094.00

Acerca de este artículo

UNSPSC Code:
12352200
NACRES:
NA.25
eCl@ss:
34058011

MXP 4,572.00


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form

lyophilized powder

Quality Segment

usage

 vial sufficient for 200 applications (on 10 cm × 10 cm mini gels)

mol wt

6,500-180,000 Da

packaging

vial of 0.1 mg protein (also contains 0.1 mg NaCl and 0.1 mg sucrose)

storage temp.

−20°C

Application

For SDS-PAGE and protein transfer, the Biotinylated Molecular Weight Standard Mixture contains proteins, which have been biotin conjugated for use as SDS-PAGE standards for the estimation of the molecular weights of blotted proteins.[1] Visualization is accomplished using streptavidin-peroxidase (Cat. No. S2438) and a color development reagent such as 4-Chloro-1-naphthol (Cat. No. C8890), eliminating the need to cut out separate lanes for general protein stains and can be performed simultaneously with immunostaining procedures. These standards may be used with either the Laemmli[2] or the Weber and Osborn[3] SDS-PAGE procedures.

Preparation Note

Each vial is reconstituted with 1 mL of 1x sample buffer (Cat. No. S3401) and incubated for 2-3 minutes at 100 °C.

Other Notes

Contains nine biotinylated proteins (approx 0.1 mg total).
This product contains:
  • α2-macroglobulin (equine serum)
  • β-galactosidase (E. coli)
  • phosphorylase b (rabbit muscle)
  • catalase (bovine liver)
  • alcohol dehydrogenase (yeast)
  • carbonic anhydrase (bovine erythrocyte)
  • trypsin inhibitor (soy bean)
  • lysozyme (chicken egg)
  • aprotinin (bovine lung)
Each vial contains approximately 33% protein, 33% NaCl and 33% sucrose.

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Este artículo
M3546SDS7B2C6210
form

lyophilized powder

form

liquid

form

-

form

liquid

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

200

Quality Level

100, 200

Quality Level

200, 400

Quality Level

100, 200

packaging

vial of 0.1 mg protein (also contains 0.1 mg NaCl and 0.1 mg sucrose)

packaging

vial of 200 μL

packaging

-

packaging

-

mol wt

6,500-180,000 Da

mol wt

-

mol wt

26,600-180,000 Da

mol wt

-

usage

 vial sufficient for 200 applications (on 10 cm × 10 cm mini gels)

usage

 vial sufficient for 400 applications on large gels (16 × 18 cm),  vial sufficient for 800 mini-gels (10 × 10 cm)

usage

-

usage

sufficient for 20 applications (on 10 cm × 10 cm mini gels)


Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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Número de artículo de comercio global

SKUGTIN
B2787-5X1VL04061833427118
B2787-1VL04061833427101

Questions

1–3 of 3 Questions  
  1. Can I see a picture of this marker? I would like to use it for chemiluminescent wester blotting

    1 answer
    1. Gel images are not provided on a lot-to-lot basis. However, historical images are available below. In this example, the marker appears in the middle two lanes.

      Helpful?

  2. The data sheets for Products SDS7B2 and B2787 do not provide a specific recommendation on how long the solutions of these molecular weight marker mixtures can be kept frozen at -20°C.  What information can you provide?

    1 answer
    1. Our R&D group recommends that after reconstitution, the solutions should be divided into aliquots and immediately frozen; avoid repeated freeze / thaw cycles. The aliquots should last at least a year at -20°C. Storage at -80°C would even be better, if it is convenient.

      Helpful?

  3. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

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