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Merck

A3151

Avidin–Peroxidase

lyophilized powder

Sinónimos:

Avidin-POD

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1 MG

MXP 2,991.00

2 MG

MXP 5,483.00

5 MG

MXP 10,312.00

MXP 2,991.00

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UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00130587
Form:
lyophilized powder
Biological source:
avidin from egg white, enzyme from horseradish

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biological source

avidin from egg white, enzyme from horseradish

conjugate

peroxidase conjugate

form

lyophilized powder

composition

Protein, ≥70% E1%/280

extent of labeling

0.7-2.0 mol peroxidase per mol avidin

technique(s)

direct ELISA: 1:50,000

solubility

H2O: soluble 1 mg/mL, PBS: soluble

storage temp.

−20°C

Quality Level

200

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1 of 4

Este artículo
A7294A2050A8706
Avidin–Peroxidase lyophilized powder

Sigma-Aldrich

A3151

Avidin–Peroxidase

Avidin–Alkaline Phosphatase buffered aqueous solution

Sigma-Aldrich

A7294

Avidin–Alkaline Phosphatase

Avidin–FITC from egg white buffered aqueous solution

Sigma-Aldrich

A2050

Avidin–FITC from egg white

Avidin from egg white recombinant, expressed in corn, ≥12 units/mg protein (E1%/280)

Sigma-Aldrich

A8706

Avidin from egg white

biological source

avidin from egg white, enzyme from horseradish

biological source

avidin from egg white, enzyme from bovine (calf) intestine

biological source

-

biological source

-

technique(s)

direct ELISA: 1:50,000

technique(s)

direct ELISA: 1:70,000, western blot: 1:150,000-1:300,000 using using β-actin in total cell extract of HeLa cells (5-10 μg per lane

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 20-40 μg/mL, indirect immunofluorescence: 1:200

technique(s)

ELISA: suitable, immunoblotting: suitable, immunoelectrophoresis: suitable

form

lyophilized powder

form

buffered aqueous solution

form

buffered aqueous solution

form

powder

solubility

H2O: soluble 1 mg/mL, PBS: soluble

solubility

-

solubility

-

solubility

-

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

conjugate

peroxidase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

FITC conjugate

conjugate

-

General description

Avidin is homotetrameric protein (68kDa) that is obtained from egg whites. This egg protein binds strongly to biotin. Thus, avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues[1]. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.

Application

Avidin-Peroxidase has been used for detecting anti-5-bromodeoxyuridine (anti-BrdU) binding[2] and OVA-IgG1[3].
To detect Ga1-deficient IgA1 antibodies in mouse serum, ELISA assays were performed using sera which was incubated with biotinylated HAA, which binds IgA1, in 96-well plates. HRP-conjugated avidin was then incubated with the sera for 40 minutes at 37 degrees.

Physical form

Lyophilized powder containing citrate buffer.

Preparation Note

Labeled with Type VI peroxidase. Coupled by a modification of the method of Sullivan, M.J., et al., FEBS Lett., 95, 311 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
SLCM5375
SLCD1995
SLCB6957
SLBW7331
SLBV8230

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Autophagy plays a key role in the pathophysiology of schizophrenia as manifested by a 40% decrease in BECN1/Beclin 1 mRNA in postmortem hippocampal tissues relative to controls. This decrease was coupled with the deregulation of the essential ADNP (activity-dependent neuroprotector
Anton B Iliuk et al.
Current protocols in chemical biology, 7(1), 17-25 (2015-03-03)
This article describes methods for a new, non-antibody phosphorylation detection reagent, termed pIMAGO (phospho-imaging). This novel reagent takes advantage not only of the unique properties of the soluble nanoparticles, but also of the multiple functionalities of the molecule, allowing for
D G Alleva et al.
Diabetes, 49(7), 1106-1115 (2000-07-26)
Cytokines derived from macrophages (Mø) play a critical role in the development of type 1 diabetes in the nonobese diabetic (NOD) mouse. Based on earlier findings from lupus-prone strains of inherent cytokine defects in Mø , NOD Mø were evaluated
Luigia Pace et al.
Journal of immunology (Baltimore, Md. : 1950), 184(11), 5969-5979 (2010-04-30)
Type I IFNs are central to a vast array of immunological functions. Their early induction in innate immune responses provides one of the most important priming mechanisms for the subsequent establishment of adaptive immunity. The outcome is either promotion or
P Birner et al.
The American journal of pathology, 158(6), 1991-1996 (2001-06-08)
Monoclonal antibody MIB-1 is a reliable tool for determining proliferating cells in human tissues, but does not react with the homologous mouse antigen and is therefore useless in experimental pathology using mice as model systems. Standard method for assessment of
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