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19899

CHAPS 100 mM solution

Sinónimos:

CHAPS solution

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Tamaño de envaseSKUDisponibilidadPrecio
1 mL
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MXP 1,652.00

Acerca de este artículo

Fórmula empírica (notación de Hill):
C32H58N2O7S
Número CAS:
Peso molecular:
614.88
NACRES:
NA.25
PubChem Substance ID:
UNSPSC Code:
12161902
MDL number:

MXP 1,652.00


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description

zwitterionic

Quality Level

form

liquid

mol wt

614.88 g/mol

concentration

100 mM in H2O

technique(s)

protein quantification: suitable

CMC

8 mM

transition temp

cloud point >100 °C

density

1.01 g/mL at 20 °C

storage temp.

2-8°C

SMILES string

C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)CC4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C

InChI

1S/C32H58N2O7S/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41)/t21-,22?,23-,24-,25+,26+,27-,28+,30+,31+,32-/m1/s1

InChI key

UMCMPZBLKLEWAF-RFCNGIAKSA-N

General description

3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS) is a biocompatible surfactant. It is a derivative of cholic acid comprising a hydrophobic cholesterol-like skeleton possessing three hydroxyl groups and a zwitterionic aliphatic tail.[1]

Application

CHAPS 100 mM solution has been used as a component of lysis buffer for the extraction of proteins from goat endometrial epithelial cells (EECs)[2] and liver tissue.[3]

Biochem/physiol Actions

3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS) exhibits the properties of both sulfobetaine-type surfactants and bile salts. It is involved in the protein extraction process for proteomic analysis and reconstitution of membrane proteins into lipid vesicles (proteoliposomes). CHAPS is broadly used in biochemistry for membrane protein and receptor isolation and purification. It is conveniently used in pharmaceuticals and medicines due to its biocompatibility, noncharged character, nondenaturing, and disaggregating properties.[1]

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Este artículo
CHAPS-ROC9426850500P
CHAPS 98% (from N), suitable for dialysis, solubility: >50% (Aqueous solutions)

Roche

CHAPS-RO

CHAPS

CHAPS Avanti Research™ - A Croda Brand

Avanti

850500P

CHAPS

technique(s)

protein quantification: suitable

technique(s)

dialysis: suitable

technique(s)

electrophoresis: suitable, protein quantification: suitable

technique(s)

protein quantification: suitable

form

liquid

form

crystals

form

powder

form

powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

room temp

storage temp.

−20°C

CMC

8 mM

CMC

4 mM, 6 mM (20-25°C)

CMC

-

CMC

-

mol wt

614.88 g/mol

mol wt

micellar avg mol wt 6150, 614.88 g/mol (anhydrous basis)

mol wt

614.88 g/mol (anhydrous basis)

mol wt

614.88 g/mol

density

1.01 g/mL at 20 °C

density

-

density

-

density

-


Clase de almacenamiento

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves



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RNAlater® is regarded as a potential preservation method for proteins, while its effect on bovine muscle proteins has rarely been evaluated. Bovine muscle protein samples (n = 12) collected from three tender (Warner⁻Bratzler shear force: 30.02⁻31.74 N) and three tough
James O'Connell et al.
Nature communications, 10(1), 5795-5795 (2019-12-21)
Tumour necrosis factor (TNF) is a cytokine belonging to a family of trimeric proteins; it has been shown to be a key mediator in autoimmune diseases such as rheumatoid arthritis and Crohn's disease. While TNF is the target of several
Horst Funken et al.
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The fucose binding lectin LecB affects biofilm formation and is involved in pathogenicity of Pseudomonas aeruginosa. LecB resides in the outer membrane and can be released specifically by treatment of an outer membrane fraction with fucose suggesting that it binds



Número de artículo de comercio global

SKUGTIN
19899-1ML-F04061838762924

Questions

  1. What is the stability time of this product at 2-8°C?

    1 answer
    1. This product is not assigned an expiration or retest date. Products that are robust in nature and extremely stable, in the original unopened container, are not assigned an expiration date or retest schedule. These products will have no dating reported on either the label or the Certificate of Analysis.
      Products that are not assigned an expiration or retest date are covered by a warranty period of 1 year from the date of product shipment.
      Please see the link below to review the complete Product Dating Information: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/190/408/product-dating-information-ms.pdf

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