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Merck
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10419

Sigma-Aldrich

8-Anilino-1-naphthalenesulfonic acid hemimagnesium salt hydrate

for fluorescence, ≥95.0% (T)

Sinónimos:

1,8-ANS magnesium salt, ANSA magnesium salt, Magnesium 8-anilino-1-naphthalenesulfonate hydrate, N-Phenyl peri acid magnesium salt

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About This Item

Fórmula empírica (notación de Hill):
C16H12Mg0.5NO3S · xH2O
Número de CAS:
18108-68-4
Peso molecular:
310.49 (anhydrous basis)
Beilstein/REAXYS Number:
4834623
EC Number:
242-004-7
MDL number:
MFCD16661224
UNSPSC Code:
12171500
PubChem Substance ID:
329749053
NACRES:
NA.47

grade

for fluorescence

Quality Level

200

assay

≥95.0% (T)

form

powder

solubility

hot water: 50 mg/mL, green

λmax

465-475 nm

fluorescence

λex 388 nm; λem 470 nm in 0.1 M Tris, 0.2 M KCl, pH 9.0, BSA

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

O.[Mg++].[O-]S(=O)(=O)c1cccc2cccc(Nc3ccccc3)c12.[O-]S(=O)(=O)c4cccc5cccc(Nc6ccccc6)c45

InChI

1S/2C16H13NO3S.Mg.H2O/c2*18-21(19,20)15-11-5-7-12-6-4-10-14(16(12)15)17-13-8-2-1-3-9-13;;/h2*1-11,17H,(H,18,19,20);;1H2/q;;+2;/p-2

InChI key

FBANPIUXAXTAFX-UHFFFAOYSA-L

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General description

ANSA (8-Anilino-1-naphthalenesulfonic acid) is a hydrophobic polarity sensitive fluorescent dye useful as a site probe to detect conformational changes in cell and micelle membranes and molecules such as proteins. ANSA is also used as a fluorescent probe for the estimation of the critical micelle concentration (CMC) of surfactants.

Features and Benefits

Fluorescent probe for the estimation of the CMC of surfactants.

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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M Bertsch et al.
Analytical biochemistry, 313(2), 187-195 (2003-02-28)
Hydrophobic sites on the surface of protein molecules are thought to have important functional roles. The identification of such sites can provide information about the function and mode of interaction with other cellular components. While the fluorescence enhancement of polarity-sensitive
A fluorimetric method for the estimation of the critical micelle concentration of surfactants.
E De Vendittis et al.
Analytical biochemistry, 115(2), 278-286 (1981-08-01)
F-O McDuff et al.
Biochemistry and cell biology = Biochimie et biologie cellulaire, 82(2), 305-313 (2004-04-03)
Canavalia ensiformis (jack bean) alpha-urease is a hexameric protein characterized by a complex denaturation mechanism. In previous papers, we have shown that a hydrophobic 8-anilino-1-naphthalenesulfonic acid (ANSA) binding conformer could be populated in a moderate concentration of denaturant. This state
Rapid fluorescent "staining" of nondenatured protein bands in agar and polyacrylamide gels.
S T Nerenberg et al.
Analytical biochemistry, 43(2), 564-574 (1971-10-01)
C D Peters et al.
Biochemistry and cell biology = Biochimie et biologie cellulaire, 75(1), 55-61 (1997-01-01)
Protein aggregation is believed to be due to conformers that expose hydrophobic clusters that promote protein association. Such conformers can be detected using a fluorescent probe like 8-anilino 1-naphthalenesulfonic acid (ANSA). Here we show that urease exposed to 1.0 M
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