10080

α-Amylase from hog pancreas

powder, ~50 U/mg

• Sinónimos: α-Amylase Enzyme
• UNSPSC Code: 12352204
• EC Number: 232-565-6
• NACRES: NA.54
• Specific activity: ~50 U/mg
• Biological source: hog pancreas

Propiedades

• biological source: hog pancreas
• Quality Segment: 100
• form: powder
• specific activity: ~50 U/mg
• storage temp.: −20°C

Descripción

General description

Pancreatic α-amylase is produced by the pancreatic acinar cells and released into the duodenum. It is a major molecule of pancreatic fluid.[1]

Application

α-Amylase from hog pancreas has been used:

• in the in vitro digestion system to study the hydrolysis of pea globulins[2]
• in vitro α-amylase inhibition study[3]
• to prepare digestive juice (salivary juice) for the in vitro model of the human digestion system[4]

Biochem/physiol Actions

α-Amylase is a starch hydrolase, for glucose production, important for energy acquisition.[5] It catalyzes the hydrolysis of α-1,4-glycosidic linkages in starch to form glucose, maltose, and maltotriose units.[1] In mammals, α-amylase regulates the sugar assimilation by reducing the glucose uptake by Na⁺/glucose cotransporter 1 (SGLT1) and stimulating glycoprotein N-glycans mediated starch digestion. α-Amylase inhibitors are associated with the treatment of type 2 diabetes mellitus.[1] α-Amylases are widely known industrial enzymes used in food, detergent, textile, fermentation, and pharmaceutical industries.[1]

Other Notes

1 U corresponds to the amount of enzyme which liberates 1 μmol maltose per minute at pH 6.9 and 25°C (starch acc. to Zulkowsky, Cat. No. 85642, as substrate)

Optimum pH and temperature 6.9 and 53 °C, respectively; calcium is required for activity and also prevents degradation by trypsin; Characterization.

Información de seguridad

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Clase de almacenamiento: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Faceshields, Gloves