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S2002

Acida sódica

ReagentPlus®, ≥99.5%

Sodium azide

Sinónimos:

Ácido hidrazoico sodium salt

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Fórmula lineal:
NaN3
Número CAS:
Peso molecular:
65.01
NACRES:
NA.21
PubChem Substance ID:
UNSPSC Code:
12352201
EC Number:
247-852-1
MDL number:
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biological source

synthetic (inorganic)

Quality Segment

product line

ReagentPlus®

assay

≥99.5%

form

powder

storage condition

(Tightly closed. Dry. Keep in a well-ventilated place. Do not store near acids.)

technique(s)

cell based assay: suitable, immunohistochemistry: suitable

color

white

mp

370-425 °C

solubility

soluble 65 g/L at 20 °C (completely)

suitability

suitable for chromatography, suitable for derivatization

application(s)

sample preparation

SMILES string

[Na]N=[N+]=[N-]

InChI

1S/N3.Na/c1-3-2;/q-1;+1

InChI key

PXIPVTKHYLBLMZ-UHFFFAOYSA-N

General description

La azida sódica (NaN3) es un potente inhibidor – un polvo cristalino hidrosoluble inodoro. Ampliamente reconocido por sus aplicaciones versátiles, desempeña un papel fundamental en la biología molecular, el cultivo celular y la investigación bioquímica. También es un inhibidor metabólico, que inhibe la fosforilación oxidativa. En cultivo celular, mantiene la integridad de sus líneas celulares.

Application

La azida sódica es adecuada para utilizar en histopatología con objeto de preparar y conservar muestras de tejido.[1] [2] También se utiliza como componente del tampón de tinción (tampón HEPES) para el inmunomarcaje de montaje entero.
Catalizador para:

  • Descarboxilación oxidativa
  • Reacciones de adición de Michael[3]

Reactivo para la síntesis de
  • copolímeros fluorescentes azules
  • Fosfonatos metálicos[4]
  • Arenos vía aminaciones
Los acetatos de Baylis-Hillman, como el etilo 2-(acetoxifenilmetil)acrilato, pueden experimentar desplazamiento nucleofílico por la acida sódica en medio acuoso para formar etilo (E)-2-azidometil-3-fenilpropenoato.

Biochem/physiol Actions

La azida sódica se usa clínicamente como conservante en el líquido de dilución para el recuento de los eritrocitos.[5] Evita el taponamiento y la internalización de anticuerpos fluorescentes ligados a la superficie en diversas aplicaciones científicas, entre ellas la citometría de flujo.

Other Notes

Si desea más información sobre nuestra gama de productos bioquímicos, cumplimente este formulario.

Legal Information

ReagentPlus is a registered trademark of Merck KGaA, Darmstadt, Germany


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Danger

Hazard Classifications

Acute Tox. 1 Dermal - Acute Tox. 2 Inhalation - Acute Tox. 2 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - STOT RE 2 Oral

target_organs

Brain

supp_hazards

Clase de almacenamiento

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

flash_point_f

Not applicable

flash_point_c

Not applicable



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Protocolos

Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.

Antibody purification protocols yield preparations containing endogenous IgG alongside specific antibodies.

Contenido relacionado


Sodium azide, a bacteriostatic preservative contained in commercially available laboratory reagents, influences the responses of human platelets via the cGMP/PKG/VASP pathway.
Russo I, et al.
Clinical Biochemistry, 41(4), 343-349 (2008)
A critical review of published methods for analysis of red cell antigen-antibody reactions by flow cytometry, and approaches for resolving problems with red cell agglutination
Patricia A Arndt , George Garratty
Transfusion Medicine Reviews, 24(3), 174-192 (2010)
Nucleophilic displacement by azide and cyanide on Baylis-Hillman acetates in water.
Yadav JS, et al.
Tetrahedron Letters, 46(16), 2761-2763 (2005)



Número de artículo de comercio global

SKUGTIN
S2002-2KG04061836916053
S2002-25KG04061833512913
S2002-500G04061835549306
S2002-25G04061835549290
S2002-100G04061835549283
S2002-5G04061835549313

Questions

1–8 of 8 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. what do you mean by Bioxtra and reagentplus? How much difference are there between two grades.

    1 answer
    1. Products that fall under the BioXtra portfolio undergo stringent testing protocols that may include trace metal analysis and specialized suitability testing, such as hybridoma or mouse embryo culture. These products include application-specific formulations such as tablets and prepared solutions.

      Products that fall under the ReagentPlus portfolio are designed for general laboratory use and have purities that are greater or equal to 95%.

      Helpful?

  4. How can I determine the concentration of sodium azide in solution?

    1 answer
    1. Sodium azide forms a 3,5-dinitrobenzoyl derivative that can be quantified by liquid chromatography.  This method is reported in the Journal of Chromatography, 5, 597 (1982).

      Helpful?

  5. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  6. How does Product S2002, Sodium azide, inhibit certain metalloenzymes?

    1 answer
    1. The mechanism of sodium azide inhibition may be due to metal ion complexation and displacement from the enzyme.

      Helpful?

  7. Are there any hazards associated with the disposal of Product S2002, Sodium azide?

    1 answer
    1. Sodium azide can form metal azides.  If you have metal pipes for your plumbing, you should not dispose of solutions down them.

      Helpful?

  8. How stable are solutions of Product S2002, Sodium azide?

    1 answer
    1. At a neutral pH sodium azide solutions are fairly stable.  Under acidic conditions it is converted to hydrazoic acid, a volatile gas.

      Helpful?

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