ESGRO® Complete Serum-free Cell Culture Freezing Medium is used along with mouse embryonic stem cells cultured in serum-free conditions with ESGRO Complete PLUS Clonal Grade Medium (Cat. Nos. SF001-100P or SF001-500P). The optimized formulation allows for consistent cryopreservation and high viability upon thawing.
Application
ESGRO® Complete Serum-Free Cell Culture Freezing Medium 50 mL has been used in cryopreservation of mouse embryonic stem (ES) cells cultured in serum-free conditions.
Legal Information
ESGRO is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Clase de almacenamiento
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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The cell culture protocols described in this instructional manual include the in vitro culture of murine ES cells using EmbryoMax products along with ESGRO® mLIF medium supplement, as well as feeder-free and serum-free ES cell culture using the ESGRO Complete™ line of products. Also included in this instructional manual are: methods for serum-free neuronal differentiation of mouse ES cells, iPS cell generation using STEMCCA™ lentivirus kits, cre-recombinase mediated excision of STEMCCA™ genes from iPS cells, derivation and rescue of new and existing ES cell lines using RESGRO™ Culture Medium and ESGRO Complete™ system.