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RAB0331

Human PSA-total ELISA Kit

Name: Human PSA-total ELISA Kit
Brand: SIGMA

for serum, plasma, cell culture supernatant and urine

Synonyme(s) :

Gamma-seminoprotein), Kallikrein-3, Prostate-specific antigen

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Conditionnement	Référence	Disponibilité	Prix

A propos de cet article

NACRES:

NA.32

UNSPSC Code:

41116158

Gene information:

human ... KLK3(354)

Input:

sample type serum
sample type urine
sample type plasma
sample type cell culture supernatant(s)

Species reactivity:

human

Storage temp.:

−20°C

Shipped in:

wet ice

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Propriétés

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable, capture ELISA: suitable

input

sample type serum
sample type urine
sample type plasma
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 8 pg/mL
standard curve range: 10.24-2500 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... KLK3(354)

Description

General description

The Human PSA-total (Prostate Specific Antigen, total) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme- linked immunosorbent assay for the quantitative measurement of human PSA-total in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human PSA

Application

For research use only. Not for use in diagnostic procedures.
Human PSA-total ELISA Kit has been used to determine PSA (prostate specific antigen) level in serum of prostate cancer patients and cancer-free patients.

Biochem/physiol Actions

Prostate-specific antigen (PSA) is an androgen-regulated serine protease specifically produced by prostatic tissue and is secreted into the lumen. PSA in semen cleaves semenogelins present in the seminal coagulum. Total PSA levels are increased in serum of prostate cancer (PCa) patients. PSA functions as a potential biomarker for PCa.[1] Serum protease inhibitors such as α1-antichymotrypsin (α1-ACT), α2-macroglobulin (α2-M), and other acute-phase proteins form irreversible complexes with circulating PSA and hinder proteolytic activity of PSA.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

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Cet article	RAB0411	RAB0517	RAB0330
Sigma-Aldrich RAB0331 Human PSA-total ELISA Kit	Sigma-Aldrich RAB0411 Human CEA ELISA Kit	Sigma-Aldrich RAB0517 Human XIAP ELISA Kit	Sigma-Aldrich RAB0330 Human SCF ELISA Kit
Gene Information human ... KLK3(354)	Gene Information human ... PSG2(5670)	Gene Information human ... XIAP(331)	Gene Information human ... KITLG(4254)
species reactivity human	species reactivity human	species reactivity human	species reactivity human
input sample type serum sample type urine sample type plasma sample type cell culture supernatant(s)	input sample type plasma sample type urine sample type serum sample type cell culture supernatant(s)	input sample type cell culture supernatant(s) sample type urine sample type serum sample type plasma	input sample type serum sample type urine sample type cell culture supernatant(s) sample type plasma
assay range inter-assay cv: <12% intra-assay cv: <10% sensitivity: 8 pg/mL standard curve range: 10.24-2500 pg/mL	assay range inter-assay cv: <12% intra-assay cv: <10% sensitivity: 200 pg/mL standard curve range: 0.343-250 ng/mL	assay range inter-assay cv: <12% intra-assay cv: <10% sensitivity: 250 pg/mL standard curve range: 2.46-600 ng/mL	assay range inter-assay cv: <12% intra-assay cv: <10% sensitivity: 2 pg/mL standard curve range: 2.74-2000 pg/mL
detection method colorimetric	detection method colorimetric	detection method colorimetric	detection method colorimetric
technique(s) ELISA: suitable, capture ELISA: suitable	technique(s) ELISA: suitable, capture ELISA: suitable	technique(s) ELISA: suitable, capture ELISA: suitable	technique(s) ELISA: suitable, capture ELISA: suitable