Product No. C2506 is prepared with trichloroacetic acid (TCA), whereas C7752 is prepared with the milder acetic acid. The TCA method may reduce the amount of superoxide dismutase (SOD) present, but tends to cause dimerization or acid-modified structures of cytochrome c. In contrast, acetic acid preparations may have slightly higher amounts of SOD, but a lower proportion of dimeric cytochrome c. So C2506 would be recommended for enzyme activity studies that require low SOD, whereas C7752 is recommended for structural studies or binding studies, or for enzyme activity studies where possible SOD contamination is not a problem. Product No. C2867 is prepared from C2506; it is a BioUtra grade product, greater than or equal to 99% pure as determined by SDS-PAGE. It is recommended when an ultra-high purity is required. Product No. C3256 is also prepared from C7752; it is isolated by ion-exchange chromatography. This fraction shows a high rate of ascorbic acid oxidation, but low activity in a cytochrome oxidase system. Product No. C7150 is also prepared from C7752; it is suitable for use as a gel filtration molecular weight marker.
C7752
Cytochrome c from equine heart
≥95% based on Mol. Wt. 12,384 basis
Synonyme(s) :
Cytochrome c from horse heart
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| Conditionnement | Référence | Disponibilité | Prix |
|---|
A propos de cet article
biological source
horse heart
Quality Segment
assay
≥95% (SDS-PAGE), ≥95% based on Mol. Wt. 12,384 basis
form
powder
mol wt
12,384
technique(s)
cell culture | mammalian: suitable
suitability
suitable for molecular biology
UniProt accession no.
storage temp.
−20°C
Gene Information
horse ... CYCS(100053958)
Application
Le cytochrome c peut entrer dans la composition d'un tampon phosphate destiné à la détection et à l'évaluation de l'activité du complexe IV par électrophorèse sur gel en conditions natives avec du bleu brillant de Coumassie (Blue Native PAGE ou BN-PAGE) Il est aussi possible de l'utiliser pour détecter des anions superoxyde extracellulaires (ECSA) dans des phagocytes rénaux isolés.
Biochem/physiol Actions
Preparation Note
Other Notes
1 of 1
Cet article | |||
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell based assay: suitable | technique(s) cell based assay: suitable | technique(s) - |
| biological source horse heart | biological source horse heart | biological source bovine heart | biological source - |
| assay ≥95% (SDS-PAGE), ≥95% based on Mol. Wt. 12,384 basis | assay ≥95% (SDS-PAGE) | assay ≥95% based on Mol. Wt. 12,327 basis | assay - |
| suitability suitable for molecular biology | suitability suitable for molecular biology | suitability suitable for molecular biology | suitability - |
| form powder | form powder | form powder or crystals | form powder |
| mol wt 12,384 | mol wt 12,384 | mol wt 12327 Da | mol wt ~12,400 |
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Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Contenu apparenté
Datasheet
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| C7752-50MG | 04061835518036 |
| C7752-500MG | 04061835556250 |
| C7752-5G | 04061835512324 |
| C7752-1G | 04061835556243 |
| C7752-100MG | 04061835556236 |
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I see that you list several horse heart cytochrome c products. What are the differences, and which one should I choose?
1 answer-
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How do I determine how much of Product C7752, Cytochrome c, is in the oxidized form, and how much is reduced?
1 answer-
This is determined by a spectral assay. The detailed assay procedure (operating procedure #11252) may be requested from Sigma St. Louis Technical Service ([email protected]).
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How should I store solutions of Product C7752, Cytochrome c?
1 answer-
Our general recommendations are as follows: Store at -20 °C (freezer) for up to six months. Store at 2-8 °C (refrigerator) for up to two weeks. Store at 20-25 °C (room temperature) for up to three days. According to J. Biol. Chem, 237, 2161-2174 (1962), cytochrome c is stable to boiling, and over an unusually wide range of pH values. However, it denatures readily at extreme pH (low or high), and in high concentrations of organic solvents, such as ethanol or acetone.
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Is Product C7752, Cytochrome c, predominantly in the oxidized form or the reduced form?
1 answer-
This product is predominantly (>95%) in the oxidized form.
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