The material should be soluble in ethanol, with dichloromethane (DCM) being a commonly used solvent for it.
37291
Dihydroethidium
BioReagent, suitable for fluorescence, ≥95% (HPCE)
Synonyme(s) :
2,7-Diamino-10-ethyl-9-phenyl-9,10-dihydrophenanthridine, 3,8-Diamino-5,6-dihydro-5-ethyl-6-phenylphenanthridine, Hydroethidine
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A propos de cet article
Formule empirique (notation de Hill) :
C21H21N3
Numéro CAS:
Poids moléculaire :
315.41
UNSPSC Code:
12161505
NACRES:
NA.32
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
5107482
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BioReagent
Quality Segment
assay
≥95% (HPCE)
form
solid
mol wt
315.41 g/mol
solubility
acetonitrile: soluble, methanol: soluble
fluorescence
λex 358 nm; λem 461 nm, λex 392 nm; λem 410 nm in methanol
suitability
suitable for fluorescence
storage temp.
−20°C
SMILES string
CCN1C(c2ccccc2)c3cc(N)ccc3-c4ccc(N)cc14
InChI
1S/C21H21N3/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14/h3-13,21H,2,22-23H2,1H3
InChI key
XYJODUBPWNZLML-UHFFFAOYSA-N
Application
Dihydroethidium (dHEt), a fluorescene probe, is used to detect the presence of reactive oxygen species (ROS) such as superoxides.
Redox indicator. Blue fluorescence until oxidized to ethidium.
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
Other Notes
B-ring reduced analog of ethidium
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Cet article | |||
|---|---|---|---|
| suitability suitable for fluorescence | suitability - | suitability suitable for fluorescence | suitability - |
| fluorescence λex 358 nm; λem 461 nm, λex 392 nm; λem 410 nm in methanol | fluorescence λex 370 nm; λem 420 nm (for cytoplasm of living cells), λex 535 nm; λem 610 nm (for chromatin of living cells) | fluorescence λex 367 nm; λem 445 nm in 0.1 M phosphate pH 7.0 (after derivatization with pyruvate) | fluorescence λex 360 nm; λem 590 nm in PBS, λex 526 nm; λem 605 nm (10 mM TBE; pH 8.0;), λex 526 nm; λem 605 nm in aqueous buffer |
| form solid | form powder | form solid | form powder |
| solubility acetonitrile: soluble, methanol: soluble | solubility chloroform: 20 mg/mL | solubility - | solubility - |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 200 |
| assay ≥95% (HPCE) | assay ≥95% | assay ≥97.0% (HPLC) | assay - |
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Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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