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Merck
  • High-throughput chemical modification of oligonucleotides for systematic structure-activity relationship evaluation.

High-throughput chemical modification of oligonucleotides for systematic structure-activity relationship evaluation.

Bioconjugate chemistry (2014-11-15)
Daniel Zewge, Francis Gosselin, Denise M Kenski, Jenny Li, Vasant Jadhav, Yu Yuan, Sandhya S Nerurkar, David M Tellers, W Michael Flanagan, Ian W Davies
KIVONAT

Chemical modification of siRNA is achieved in a high-throughput manner (96-well plate format) by copper catalyzed azide-alkyne cycloadditions. This transformation can be performed in one synthetic operation at up to four positions with complete specificity, good yield, and acceptable purity. As demonstrated here, this approach extends the current synthetic options for oligonucleotide modifications and simultaneously facilitates the systematic, rapid biological evaluation of modified siRNA.

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Termékleírás

Supelco
Water, for TOC analysis
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Triethylamine, ≥99.5%
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Methanol, NMR reference standard
Supelco
Water, suitable for ion chromatography
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Methylamine solution, 2.0 M in methanol
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Imidazole, puriss. p.a., ≥99.5% (GC)
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Triethylamine, ≥99%
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Water, sterile-filtered, BioReagent, suitable for cell culture
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Water, PCR Reagent
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Trichloroacetic acid, ≥99.0% (titration)
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Trichloroacetic acid, suitable for electrophoresis, suitable for fixing solution (for IEF and PAGE gels), ≥99%
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E-Toxate Water, endotoxin, free
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Imidazole, for molecular biology, ≥99% (titration)
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Imidazole, ≥99% (titration), crystalline
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Triethylamine, ≥99.5%
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Imidazole, ACS reagent, ≥99% (titration)
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2,6-Lutidine, ReagentPlus®, 98%
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Methylamine solution, 2.0 M in THF
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Trichloroacetic acid, ACS reagent, for the determination of Fe in blood according to Heilmeyer, ≥99.5%
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Trichloroacetic acid, BioUltra, ≥99.5% (T)
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Water, for cell biology, sterile ultrafiltered
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Water, for molecular biology, sterile filtered
Supelco
Water, for HPCE, for luminescence, suitable for UV/Vis spectroscopy
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Triethylamine, puriss. p.a., ≥99.5% (GC)
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Triethylamine, for amino acid analysis, ≥99.5% (GC)
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Water, tested according to Ph. Eur.
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Triethylamine, BioUltra, ≥99.5% (GC)
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Azidomethyl phenyl sulfide, 95%
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Methylamine solution, 40 wt. % in H2O
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2,6-Lutidine