Ugrás a tartalomra
Merck
  • High-throughput chemical modification of oligonucleotides for systematic structure-activity relationship evaluation.

High-throughput chemical modification of oligonucleotides for systematic structure-activity relationship evaluation.

Bioconjugate chemistry (2014-11-15)
Daniel Zewge, Francis Gosselin, Denise M Kenski, Jenny Li, Vasant Jadhav, Yu Yuan, Sandhya S Nerurkar, David M Tellers, W Michael Flanagan, Ian W Davies
KIVONAT

Chemical modification of siRNA is achieved in a high-throughput manner (96-well plate format) by copper catalyzed azide-alkyne cycloadditions. This transformation can be performed in one synthetic operation at up to four positions with complete specificity, good yield, and acceptable purity. As demonstrated here, this approach extends the current synthetic options for oligonucleotide modifications and simultaneously facilitates the systematic, rapid biological evaluation of modified siRNA.

ANYAGOK
Cikkszám
Márka
Termékleírás

Sigma-Aldrich
Benzyl azide solution, ~0.5 M in dichloromethane, ≥95.0% (HPLC)
Supelco
Water, for TOC analysis
Supelco
Water, suitable for ion chromatography
Sigma-Aldrich
Methanol, NMR reference standard
Sigma-Aldrich
Triethylamine, ≥99.5%
Sigma-Aldrich
Water, for cell biology, sterile ultrafiltered
Sigma-Aldrich
Water, for molecular biology, sterile filtered
Supelco
Water, for HPCE, for luminescence, suitable for UV/Vis spectroscopy
Sigma-Aldrich
Triethylamine, puriss. p.a., ≥99.5% (GC)
Sigma-Aldrich
Triethylamine, for amino acid analysis, ≥99.5% (GC)
Sigma-Aldrich
Water, tested according to Ph. Eur.
Sigma-Aldrich
2,6-Lutidine
Sigma-Aldrich
Triethylamine, BioUltra, ≥99.5% (GC)
Sigma-Aldrich
2,6-Lutidine, ReagentPlus®, 98%
Sigma-Aldrich
Azidomethyl phenyl sulfide, 95%
Sigma-Aldrich
Methylamine solution, 40 wt. % in H2O
Sigma-Aldrich
Methylamine solution, 2.0 M in THF
Sigma-Aldrich
Imidazole, puriss. p.a., ≥99.5% (GC)
Sigma-Aldrich
Trichloroacetic acid, ACS reagent, for the determination of Fe in blood according to Heilmeyer, ≥99.5%
Sigma-Aldrich
Trichloroacetic acid, BioUltra, ≥99.5% (T)
Sigma-Aldrich
Imidazole, ReagentPlus®, 99%
Sigma-Aldrich
Triethylamine, for protein sequence analysis, ampule, ≥99.5% (GC)
Supelco
2,6-Lutidine, analytical standard
Sigma-Aldrich
Imidazole, BioUltra, for molecular biology, ≥99.5% (GC)
Sigma-Aldrich
Imidazole, BioUltra, ≥99.5% (GC)
Supelco
Methanol, analytical standard
Sigma-Aldrich
Triethylamine, ≥99%
Sigma-Aldrich
Water, sterile-filtered, BioReagent, suitable for cell culture
Sigma-Aldrich
Water, PCR Reagent
Sigma-Aldrich
Trichloroacetic acid, ≥99.0% (titration)