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F4890

Monoclonal Anti-Rabbit Immunoglobulins–FITC antibody produced in mouse

Name: Monoclonal Anti-Rabbit Immunoglobulins–FITC antibody produced in mouse
Brand: SIGMA
Price: 212 EUR

clone RG-16, purified immunoglobulin, buffered aqueous solution

Synonyme(s) :

Monoclonal Anti-Rabbit Immunoglobulins (IgG, IgA, IgM)

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Conditionnement	Référence	Disponibilité	Prix
0.2 mL		Vérifier la disponibilité des articles du panier	212,00 €

A propos de cet article

NACRES:

NA.46

UNSPSC Code:

12352203

Conjugate:

FITC conjugate

Clone:

RG-16, monoclonal

Application:

IF, IHC (p)

Citations:

212,00 €

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Propriétés

biological source

mouse

Quality Segment

200

conjugate

FITC conjugate

antibody form

purified immunoglobulin

antibody product type

secondary antibodies

clone

RG-16, monoclonal

form

buffered aqueous solution

should not react with

human, pig, chicken, bovine, horse, guinea pig, turkey, rat, canine, goat, feline, sheep

storage condition

protect from light

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20, indirect immunofluorescence: 1:50 using using chicken fibroblast cells and primary antibody produced in rabbit

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

Reacts with an epitope on the heavy chain of rabbit IgG, IgA and IgM, which is sensitive to reduction. No cross-reactivity with human IgG, IgA and IgM.

Application

Monoclonal Anti-Rabbit Immunoglobulins-FITC antibody produced in mouse has been used in:

immunocytochemistry
immunohistochemistry
immunofluorescence

The mouse monoclonal anti-Rabbit IgG (heavy chain-specific) peroxidase conjugated antibody can be used as a secondary antibody for western blot analysis of protein preps from E. coli cultures at a concentration of 1:10000 diluted in PBSt for 1 hour at room temperature.

Biochem/physiol Actions

Binds only rabbit Igs. Does not react with reduced rabbit Igs.

Physical form

Solution in 0.01 M sodium phosphate buffer, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Cet article	F4891	B3275	B5283
Sigma-Aldrich F4890 Monoclonal Anti-Rabbit Immunoglobulins–FITC antibody produced in mouse	Sigma-Aldrich F4891 Anti-Goat/Sheep IgG−FITC antibody, Mouse monoclonal	Sigma-Aldrich B3275 Monoclonal Anti-Rabbit Immunoglobulins–Biotin antibody produced in mouse	Sigma-Aldrich B5283 Anti-Rabbit IgG (γ-chain specific)−Biotin antibody, Mouse monoclonal
conjugate FITC conjugate	conjugate FITC conjugate	conjugate biotin conjugate	conjugate biotin conjugate
biological source mouse	biological source mouse	biological source mouse	biological source mouse
antibody form purified immunoglobulin	antibody form purified from hybridoma cell culture	antibody form purified immunoglobulin	antibody form purified immunoglobulin
technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20, indirect immunofluorescence: 1:50 using using chicken fibroblast cells and primary antibody produced in rabbit	technique(s) dot immunobinding: 1:32, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20, particle immunofluorescence: 1:32	technique(s) direct ELISA: 1:60,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,500, western blot: 1:200,000-1:400,000 using using an assay detecting actin in total cell extract of HeLa cells (5-10 μg per well)	technique(s) direct ELISA: 1:150,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000, western blot (chemiluminescent): 1:200,000-1:400,000
clone RG-16, monoclonal	clone GT-34, monoclonal	clone RG-16, monoclonal	clone RG-96, monoclonal
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C

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Classe de stockage

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificats d'analyse (COA)

Lot/Batch Number

0000417623

0000324929

0000249551

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Contenu apparenté

Data Sheet - F4890

Instructions

Numéro d'article de commerce international

Référence	GTIN
F4890-.2ML	04061838354471

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