E0127
Elastase from porcine pancreas
Type III, lyophilized powder, Protein 55-85 %, ≥4.0 units/mg protein
Synonyme(s) :
Elastase from hog pancreas, Pancreatopeptidase E
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About This Item
Numéro CAS:
Numéro CE :
Numéro MDL:
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54
Produits recommandés
Type
Type III
Forme
lyophilized powder
Activité spécifique
≥4.0 units/mg protein
Poids mol.
25.9 kDa
Composition
Protein, 55-85%
Solubilité
200 mM Tris HCl buffer, pH 8.8: soluble 1.0 mg/mL, clear
Température de stockage
−20°C
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Application
Elastase from porcine pancreas has been used in a study to identify a novel bone/calcium metabolism-regulating factor in porcine pancreas. Elastase from porcine pancreas has also been used in a study to investigate the effect of a specific synthetic inhibitor of neutrophil elastase (ONO-5046) on the course of acute hemorrhagic pancreatitis in dogs.
Elastase from porcine pancreas has been used in the proteolytic cleavage of UMP synthase (Uridine-5′-monophosphate synthase). It has also been used along with collagenase to disperse tissue into single cells from the thoracic media, abdominal aortic media and intima of rabbit.
Actions biochimiques/physiologiques
Elastase is a single polypeptide chain of 240 amino acid residues and contains four disulfide bridges. The molecular mass is approximately 25.9 kDa. The enzyme is synthesized as an inactive zymogen, proelastase, which is converted to the active form by limited proteolysis at the N-terminal by trypsin. It is a serine protease with broad specificity. It cleaves protein at the carboxyl side of small hydrophobic amino acids such as Ile, Gly, Ala, Ser, Val, and Leu. The enzyme also hydrolyzes amides and esters such as N-Benzoyl-L-alanine methyl ester. The pH optimum is found to be 8.0-8.5. It does not require any activator, but it is inhibited by diisopropyl fluorophosphate, phenylmethanesulfonyl fluoride, α2-macroglobulin, α1-antitrypsin, sulfonyl fluorides, p-dinitrophenyl diethylphosphate and high salt concentrations. Elastase is extensively used in tissue and cell dissociation procedures. It is effective in the isolation of Type II lung cells.
Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin.
Conditionnement
Package size based on protein content
Qualité
Contains trypsin activity
Définition de l'unité
One unit will hydrolyze 1.0 μmole of N-succinyl-L-Ala-Ala-Ala-p-nitroanilide per min, pH 8.0 at 25 °C.
Forme physique
Contains sodium carbonate.
Notes préparatoires
2x crystallized and chromatographically purified
Application
Réf. du produit
Description
Tarif
Inhibiteur
Réf. du produit
Description
Tarif
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Organes cibles
Respiratory system
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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Les clients ont également consulté
J H Campbell et al.
Arteriosclerosis (Dallas, Tex.), 5(4), 318-328 (1985-07-01)
Smooth muscle cells of the rabbit aorta, when grown in vitro, express three distinguishable forms of phenotype (contractile, reversible synthetic, and irreversible synthetic). We compared the interactions of these three smooth muscle phenotypes with rabbit very low density lipoprotein (VLDL)
R H Kelly et al.
Digestion, 22(1), 1-7 (1981-01-01)
Induction of acute hemorrhagic pancreatic necrosis by dietary means in mice produces alterations in the serum complement system. Total hemolytic complement, i.e., CH50, and native C3 levels fall during the development of pancreatitis while, at the same time, what could
E E Floyd et al.
The Journal of biological chemistry, 260(16), 9443-9451 (1985-08-05)
The multifunctional protein uridine 5'-monophosphate (UMP) synthase catalyzes the final two reactions of the de novo biosynthesis of UMP in mammalian cells by the sequential action of orotate phosphoribosyltransferase (EC 2.4.2.10) and orotidine 5'-monophosphate (OMP) decarboxylase (EC 4.1.1.23). This protein
Ting Wang et al.
Biochemical and biophysical research communications, 485(2), 284-289 (2017-02-23)
Acute lung injury (ALI) is an early pathophysiologic change in acute respiratory distress syndrome and its management can be challenging. Omalizumab (Xolair™) is a recombinant DNA-derived, humanized antibody. OMZ-SPT is a polypeptide on the heavy chain of omalizumab monoclonal antibody.
Domenico M Guarascio et al.
The Journal of physiology, 599(15), 3697-3714 (2021-06-06)
Taste transduction occurs in taste buds in the tongue epithelium. The Ca2+ -activated Cl- channels TMEM16A and TMEM16B play relevant physiological roles in several sensory systems. Here, we report that TMEM16A, but not TMEM16B, is expressed in the apical part
Protocoles
Enzymatic Assay of Elastase (EC 3.4.21.36), a continuous spectrophotometric rate determination.
Chromatograms
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