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D6665
2′,7′-Dichlorofluorescein
~90% purity (TLC) (85- 100% purity), powder
Synonyme(s) :
Dichlorofluorescein; Fluorescein 27
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| Conditionnement | Référence | Disponibilité | Prix |
|---|---|---|---|
| 5 g | Expédition prévue aujourd'huiDeSt QuentinFallavier | 97,00 € | |
| 25 g | Vérifier la disponibilité des articles du panier | 374,00 € |
A propos de cet article
Formule empirique (notation de Hill) :
C20H10Cl2O5
Numéro CAS:
Poids moléculaire :
401.20
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
EC Number:
200-968-6
MDL number:
Beilstein/REAXYS Number:
58009
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Laissez-nous vous aiderNom du produit
2′,7′-Dichlorofluorescein, suitable for use as an indicator in chloride titration, ~90% (TLC), powder
Quality Segment
assay
~90% (TLC)
form
powder
technique(s)
titration: suitable
mp
280 °C (dec.) (lit.)
solubility
ethanol: 25 mg/mL
density
0.79 g/cm3
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
Oc1cc2Oc3cc(O)c(Cl)cc3C4(OC(=O)c5ccccc45)c2cc1Cl
InChI
1S/C20H10Cl2O5/c21-13-5-11-17(7-15(13)23)26-18-8-16(24)14(22)6-12(18)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H
InChI key
VFNKZQNIXUFLBC-UHFFFAOYSA-N
General description
2′,7′-Dichlorofluorescein is an oxidation-sensitive fluorescent probe. 2′7′-dichlorofluorescein diacetate is oxidized to 2′7′-dichlorofluorescein in the presence of ROS (reactive oxygen species).citation
Application
2′,7′-Dichlorofluorescein has been used as an oxidation-sensitive fluorescent probe to measure ROS (reactive oxygen species) formation in cells.[1]
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Cet article | |||
|---|---|---|---|
| technique(s) titration: suitable | technique(s) titration: suitable | technique(s) - | technique(s) - |
| form powder | form powder | form solid | form powder |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) - | application(s) - |
| solubility ethanol: 25 mg/mL | solubility alcohol: passes test | solubility - | solubility - |
| storage temp. room temp | storage temp. room temp | storage temp. - | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
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Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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Contenu apparenté
Vittoria Buccigrossi et al.
PloS one, 9(6), e99830-e99830 (2014-06-12)
Rotavirus (RV) infection causes watery diarrhea via multiple mechanisms, primarily chloride secretion in intestinal epithelial cell. The chloride secretion largely depends on non-structural protein 4 (NSP4) enterotoxic activity in human enterocytes through mechanisms that have not been defined. Redox imbalance
Cysteamine suppresses human peripheral blood mononuclear cells--human corneal endothelial cell reaction via reactive oxygen species reduction.
Shin YJ, et al.
Molecular Vision, 17, 3371-3378 (2011)
Andréa G K Ferreira et al.
Journal of cellular biochemistry, 113(1), 174-183 (2011-09-02)
The present study investigated the effects of chronic hyperprolinemia on oxidative and metabolic status in liver and serum of rats. Wistar rats received daily subcutaneous injections of proline from their 6th to 28th day of life. Twelve hours after the
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| D6665-5G | 04061835347520 |
| D6665-25G | 04061833587812 |
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What is the Department of Transportation shipping information for this product?
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In what types of applications can 2´,7´-Dichlorofluorescein be used?
1 réponse-
Some applications for use of 2´,7´-Dichlorofluorescein are listed below. This product can be used for the determination of carbohydrates by fluorescence densitometry after TLC. See G. Gübitz et al., J. Chromatogr., 117, 337, (1976). 2´,7´-Dichlorofluorescein as also been used in the determination of H2O2 by chemiluminescence. See the following references by H. Watanabe et al. Anal. Sci. 2, 461, (1986) and T. Segawa et al. Anal. Sci. 6, 763, (1990). This product has also been used as a fluorescent additive used in indirect fluorimetric detection in OTC-HPLC. See W.D. Pfeffer and E.S. Yeung, J. Chromatogr., 506, 401, (1990).
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What can be used to make a solution of 2´,7´-dichlorofluorescein?
1 réponse-
2´,7´-Dichlorofluorescein is soluble at 25 mg/mL absolute ethanol with heating. 2´,7´-Dichlorofluorescein is also soluble in water at 0.1 mg/mL.
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What excitation /emission wavelengths are best for fluorescence detection of 2´,7´-Dichlorofluorescein?
1 réponse-
2´,7´-Dichlorofluorescein can be detected at an excitation wavelength at 504 nm and emission wavelength at 529 nm in 0.1 M Tris pH 8.0.
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