C4237
Protamine Kinase, Cytosolic from bovine kidney
>90% (SDS-PAGE), buffered aqueous glycerol solution
Synonyme(s) :
Cytosolic Protamine Kinase
Se connecter pour consulter les tarifs organisationnels et contractuels.
Sélectionner une taille de conditionnement
Changer de vue
| À vous/Référence | Disponibilité | Prix |
|---|---|---|
1 vial | Vérifier la disponibilité des articles du panier | 802,00 € |
A propos de cet article
Specific activity:
≥15,000 units/mg protein
Assay:
>90% (SDS-PAGE)
802,00 €
Vérifier la disponibilité des articles du panier
Service technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aiderQuality Segment
assay
>90% (SDS-PAGE)
form
buffered aqueous glycerol solution
specific activity
≥15,000 units/mg protein
mol wt
45 kDa
packaging
vial of 100 ng
shipped in
dry ice
storage temp.
−70°C
Application
Cytosolic Protamine Kinase (cPK) is isolated from bovine kidney and has a molecular mass of approximately 45 kDa. It phosphorylates the eukaryotic Initiation Factor 4E (eIF4E), which initiates translation by mRNA. It is used to study protein synthesis and various cellular processes.
Biochem/physiol Actions
Cytosolic protamine kinase is involved in the regulation of protein synthesis and is indirectly associated with numerous cellular processes. Cytosolic protamine kinase is a distinct insulin-stimulated kinase involved in the phosphorylation of eukaryotic Initiation Factor 4E (eIF4E) which is key to initiating translation by mRNA. This protein appears to be inactivated by protein phosphatase 2A family members and may also be inhibited by microcystin, okadeic acid, and ATP. The phosphorylation process is reversible and MBPK1 and MBPK2 (Myelin Basic Protein Kinase 1 and 2) may reactivate cytosolic protamine kinase [1].
Physical form
Solution in 50 μl of 50 mM Tris-HCl, pH 7.0, containing 14 mM β-mercaptoethanol, 1mM benzamidine, 0.1 mM PMSF, 1mM EDTA, 50% glycerol
Other Notes
One unit will incorporate 1 nmol of phosphate using protamine sulfate as a substrate per minute at pH 7.0 at 30 °C.
1 of 1
Cet article | |||
|---|---|---|---|
| specific activity ≥15,000 units/mg protein | specific activity ≥10 nmol/min·mg | specific activity ≥1,000 units/mg protein (biuret) | specific activity ~17,000 units/mg protein |
| assay >90% (SDS-PAGE) | assay ≥80% (SDS-PAGE) | assay - | assay - |
| form buffered aqueous glycerol solution | form buffered aqueous glycerol solution | form buffered aqueous glycerol solution | form aqueous solution |
| mol wt 45 kDa | mol wt protein apparent mol wt ~65 kDa (including N-terminal GST-tag of ~26kDa) | mol wt - | mol wt 36 kDa |
| shipped in dry ice | shipped in dry ice | shipped in - | shipped in dry ice |
| storage temp. −70°C | storage temp. −70°C | storage temp. 2-8°C | storage temp. −70°C |
Classe de stockage
10 - Combustible liquids
Faites votre choix parmi les versions les plus récentes :
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.