ABC299

Anti-FFAR2 Antibody/GPR43

from rabbit, purified by affinity chromatography

• Synonyme(s) : Free fatty acid receptor 2, G-protein coupled receptor 43, FFAR2/GPR43
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IHC, WB
• Citations: 13

Propriétés

• biological source: rabbit
• Quality Level: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: rat
• species reactivity (predicted by homology): human (based on 100% sequence homology), mouse
• technique(s): immunohistochemistry: suitable, western blot: suitable
• NCBI accession no.: NP_005297
• UniProt accession no.: O15552
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... FFAR2(2867)

Description

General description

Free fatty acid receptor 2 (FFAR2), also known as G-protein coupled receptor 43 (GPR43), functions as the cell membrane receptor for short chain fatty acids that ultimately stimulates G protein coupled, inhibitory (Gi) signals via the adenylate cyclase pathway. FFAR2/GPR43 is found expressed in leukocytes in the lamina propria of the small intestine mostly but some expression can be also be found in splenocytes, adipose tissue, and enteroendocrine cells of the gut. Interesting, FFAR2/GPR43 because it senses short chain fatty acids which are often key metabolites of gut microbiota, it may serve as a biological sensor to regulate the activity of regulatory T cells and the immune response in diseases such as colitis.

~47 kDa observed. The calculated molecular weight of this protein is 37 kDa, but can be observed at ~47 kDa due to high glycosylation. Uncharacterized band(s) may appear in some lysates.

Immunogen

Epitope: Cytoplasmic domain

GST-tagged recombinant protein corresponding to the cytoplasmic domain of human FFAR2/GPR43.

Application

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-FFAR2 Antibody/GPR43 is validated for use in Western Blotting and Immunohistochemistry for the detection of FFAR2/GPR43.

Western Blotting Analysis: 1.0 µg/mL from a representative lot detected FFAR2/GPR43 in 10 µg of RAW264.7 cell lysate.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected FFAR2/GPR43 in mouse kidney and mouse liver tissue.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blotting in CHEM-1 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected FFAR2/GPR43 in 10 µg of CHEM-1 cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Classe de stockage: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable