A search of the website does not show a density for product G4155. For products supplied in small quantities, it is not common for the density to be measured. However, for solutions available in larger quantities, density information is often provided. Although the density for G4155 is not stated, there is some useful information available from the website. The physical form of the product is a suspension in 50% glycerol, 5 mM Tris buffer salts, 5 mM magnesium chloride, 0.5 mM DTT, and 0.5 mM mercaptoethanol at pH 7.4.
Összes fotó(1)
Fontos dokumentumok
G4155
β-Galactosidase from Escherichia coli
aqueous glycerol suspension, ≥500 units/mg protein (biuret)
Szinonimák:
β-D-Galactoside galactohydrolase, Lactase
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Méret kiválasztása
1000 UNITS
42 200,00 Ft
3000 UNITS
107 000,00 Ft
5000 UNITS
157 000,00 Ft
42 200,00 Ft
Az elérhetőséggel kapcsolatos kérdésekkel kérjük, forduljon Vevőszolgálatunkhoz.
Összes fotó(1)
Méret kiválasztása
Nézet módosítása
1000 UNITS
42 200,00 Ft
3000 UNITS
107 000,00 Ft
5000 UNITS
157 000,00 Ft
About This Item
CAS-szám:
MDL-szám:
UNSPSC kód:
12352204
NACRES:
NA.54
42 200,00 Ft
Az elérhetőséggel kapcsolatos kérdésekkel kérjük, forduljon Vevőszolgálatunkhoz.
Javasolt termékek
Forma
aqueous glycerol suspension
Minőségi szint
specifikus aktivitás
≥500 units/mg protein (biuret)
molekulatömeg
465 kDa
tárolási hőmérséklet
−20°C
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Related Categories
Alkalmazás
β-Galactosidase is used in the enzymatic assays in the synthesis of imidazolo-pyrrolidinoses.
Biokémiai/fiziológiai hatások
β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.
Fizikai tulajdonságok
Tetramer molecular weight 465 kDa (subunits 116.3 kDa each)
Egység definíció
One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.
Fizikai forma
Suspension in 50% glycerol, 5 mM Tris buffer salts, 5 mM magnesium chloride, 0.5 mM DTT, 0.5 mM mercaptoethanol. pH 7.4
Egyéb megjegyzések
Loss of enzymatic activity can occur if this enzyme is frozen. This enzyme is provided in a 50% glycerol solution and will not freeze if stored at -20° Celsius. It is not recommended that this enzyme preparation be stored in an ultracold freezer (-60 to -100° Celsius).
Gátló
Product No.
Leírás
Árazás
szubsztrát
Product No.
Leírás
Árazás
Tárolási osztály kódja
10 - Combustible liquids
WGK
WGK 2
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Válasszon a legfrissebb verziók közül:
Analitikai tanúsítványok (COA)
Lot/Batch Number
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Az ügyfelek ezeket is megtekintették
Théophile Tschamber et al.
Bioorganic & medicinal chemistry, 11(17), 3559-3568 (2003-08-07)
The syntheses of four glyco-imidazoles, which are pentose-derivatives belonging to the D-series, as well as the syntheses of their L-enantiomers, are reported. Starting from the known linear xylo, lyxo, arabino, and ribo imidazolo-pentoses in both the L- and the D-series
Olga Ermakova et al.
Brain sciences, 11(6) (2021-07-03)
Acquisition of detailed anatomical and molecular knowledge from intact biological samples while preserving their native three-dimensional structure is still a challenging issue for imaging studies aiming to unravel a system's functions. Three-dimensional micro-CT X-ray imaging with a high spatial resolution
Christophe Laumonnerie et al.
Development (Cambridge, England), 141(3), 594-603 (2014-01-23)
The establishment of anatomically stereotyped axonal projections is fundamental to neuronal function. While most neurons project their axons within the central nervous system (CNS), only axons of centrally born motoneurons and peripherally born sensory neurons link the CNS and peripheral
A Hinek et al.
American journal of human genetics, 67(1), 23-36 (2000-06-08)
We have previously shown that intracellular trafficking and extracellular assembly of tropoelastin into elastic fibers is facilitated by the 67-kD elastin-binding protein identical to an enzymatically inactive, alternatively spliced variant of beta-galactosidase (S-Gal). In the present study, we investigated elastic-fiber
S Zhang et al.
The Biochemical journal, 348 Pt 3, 621-632 (2000-06-07)
We have identified and characterized three missense mutations in a patient with type 1 G(M1) gangliosidosis, namely a substitution of G for A at nucleotide position 1044 (G1044-->A; in exon 10) on one allele, which converts Asp(332) into asparagine, and
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Could you please advise if the density of the aqueous suspension of beta galactosidase from Escherichia coli (G4155) can be assumed as 1 uL of solution is equal to 1 mg, given that the SDS and provided enzyme assay do not contain this information?
1 answer-
Helpful?
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We recently purchased this enzyme. Information given regarding enzyme volume is only 1000 units. I want to know what would be units per uL. How much is the liquid volume in the given product to calculate the enzyme concentration? Provide proper informatio
1 answer-
Units/ml can be calculated using the numbers reported in batch specific CofA. Below is an example.
CofA of G4155, lot 0000303635
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/G4155-3KU0000303635.pdf
1.5 mgP/ml X 766 units/mg Protein = 1149 units/mlHelpful?
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