G3153

β-Galactosidase from Escherichia coli

lyophilized powder, ≥500 units/mg protein

• Szinonimák: β-D-Galactoside galactohydrolase, Lactase
• CAS-szám: 9031-11-2
• Enzyme Commission szám: 3.2.1.23 (BRENDA, IUBMB)
• EC-szám: 232-864-1
• MDL-szám: MFCD00130623
• UNSPSC kód: 12352204
• NACRES: NA.54

Tulajdonságok

• rekombináns: expressed in E. coli
• Minőségi szint: 200
• Forma: lyophilized powder
• specifikus aktivitás: ≥500 units/mg protein
• molekulatömeg: 465 kDa
• tárolási hőmérséklet: −20°C

Leírás

Általános leírás

β-galactosidase is a 465 kDa tetrameric protein. It is coded by the LacZ gene.

Alkalmazás

β-Galactosidase from Escherichia coli has been used to produce biocatalytic Ca-alginate beads on a B-390 encapsulator (BUCHI). It has been used to prepare the source droplet solution and also as the cargo for Pep-1 (KETWWETWWTEWSQPKKKRKV-cysteamide).

β-Galactosidase may be used for derivatization, such as an enzyme label for IgG, without prior dialysis or gel filtration.

Biokémiai/fiziológiai hatások

β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.

β-galactosidase is considered as a reporter enzyme in applications involving gene expression regulation, analysis of protein function/structure and target gene expression.

Kiszerelés

Package size based on protein content

Fizikai tulajdonságok

Tetramer molecular weight 465 kDa (subunits 116.3 kDa each)

Egység definíció

One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactopyranoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.

Fizikai forma

Stabilized with phosphate buffer and sucrose

Biztonsági adatok

• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves, type N95 (US)