E1250
Elastase from porcine pancreas
Type I, ≥4.0 units/mg protein
Szinonimák:
Elastase from hog pancreas, Pancreatopeptidase E
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
CAS-szám:
EC-szám:
MDL-szám:
UNSPSC kód:
12352204
NACRES:
NA.54
Javasolt termékek
![N-[3-(2-Furyl)acryloyl]-Leu-Gly-Pro-Ala](/deepweb/assets/sigmaaldrich/product/structures/805/876/96b5fb57-71c8-4c6b-b5d2-fafe7374cd85/640/96b5fb57-71c8-4c6b-b5d2-fafe7374cd85.png)
biológiai forrás
Porcine pancreas
Minőségi szint
típus
Type I
form
suspension
specifikus aktivitás
≥4.0 units/mg protein
molekulatömeg
25.9 kDa
tartalmaz
0.1% thymol
koncentráció
0.5-15.0 mg/mL in water
idegen aktivitás
trypsin ≤50 BAEE units/mg protein
tárolási hőmérséklet
2-8°C
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Related Categories
Alkalmazás
Elastase from porcine pancreas has been used in a study to investigate the design, synthesis and evaluation of biomimetic affinity ligands for elastases. Elastase from porcine pancreas has also been used in a study to investigate the purification and partial characterization of the pancreatic proteolytic enzymes trypsin, chymotrypsin, and elastase.
Elastase from porcine pancreas has been used:
- to induce abdominal aortic aneurysm (AAA)
- to study the impact of indoleamine 2-3 dioxygenase 1 (IDO) in mice
- to digest aortas for aortic smooth muscle cells (SMC) isolation
- as a positive control of proteolytic digestion
The enzyme from Sigma has been used in the development of elastase-perfused animal model . This study determined if tobacco exposure could lower the threshold of aortic injury necessary for AAA (abdominal aortic aneurysm) development. It has also been used during the isolation of type II pneumocytes from human lungs.
Biokémiai/fiziológiai hatások
Elastase is a single polypeptide chain of 240 amino acid residues and contains four disulfide bridges. The molecular mass is approximately 25.9 kDa. The enzyme is synthesized as an inactive zymogen, proelastase, which is converted to the active form by limited proteolysis at the N-terminal by trypsin. It is a serine protease with broad specificity. It cleaves protein at the carboxyl side of small hydrophobic amino acids such as Ile, Gly, Ala, Ser, Val, and Leu. The enzyme also hydrolyzes amides and esters such as N-Benzoyl-L-alanine methyl ester. The pH optimum is found to be 8.0-8.5. It does not require any activator, but it is inhibited by diisopropyl fluorophosphate, phenylmethanesulfonyl fluoride, α2-macroglobulin, α1-antitrypsin, sulfonyl fluorides and p-dinitrophenyl diethylphosphate and high salt concentrations. It is extensively used in tissue and cell dissociation procedures. Elastase is effective in the isolation of Type II lung cells.
Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin.
Kiszerelés
Package size based on protein content
Egység definíció
One unit will hydrolyze 1.0 μmole of N-succinyl-L-Ala-Ala-Ala-p-nitroanilide per min, pH 8.0 at 25 °C.
Elkészítési megjegyzés
2× crystallized
Alkalmazás
inhibitor
Product No.
Leírás
Árazás
Figyelmeztetés
Danger
Figyelmeztető mondatok
Óvintézkedésre vonatkozó mondatok
Veszélyességi osztályok
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Célzott szervek
Respiratory system
Tárolási osztály kódja
11 - Combustible Solids
WGK
WGK 3
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Egyéni védőeszköz
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
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Az ügyfelek ezeket is megtekintették
Júlia Benini Kohler et al.
Biology open, 8(4) (2019-04-12)
Macrophages play a pivotal role in the development of emphysema and depending on the microenvironment stimuli can be polarized into M1- or M2-like macrophage phenotypes. We compared macrophage polarizations in cigarette smoke (CS)- and porcine pancreatic elastase (PPE)-induced emphysema models.
Andreas Giraud et al.
Cardiovascular research, 113(11), 1364-1375 (2017-06-06)
Abdominal aortic aneurysm (AAA), frequently diagnosed in old patients, is characterized by chronic inflammation, vascular cell apoptosis and metalloproteinase-mediated extracellular matrix destruction. Despite improvement in the understanding of the pathophysiology of aortic aneurysm, no pharmacological treatment is yet available to
A C Cunningham et al.
Journal of cell science, 107 ( Pt 2), 443-449 (1994-02-01)
Highly purified populations of alveolar epithelial cells (type II pneumocytes) were isolated from human lung specimens. These cells were characterised histochemically, by demonstrating the presence of intracellular alkaline phosphatase, and morphologically, by electron microscopic demonstration of lamellar bodies and microvilli.
Demetrios Moris et al.
Current vascular pharmacology, 16(2), 168-178 (2017-02-06)
Abdominal aortic aneurysm (AAA) formation is associated with by inflammation and matrix degradation. This study tested the hypothesis that calprotectin, a novel biomarker for inflammation, as well as established biomarkers such as C-reactive protein (CRP) and matrix metalloproteinase- 9 (MMP-9)
Endothelial MMP-9 drives the inflammatory response in abdominal aortic aneurysm (AAA)
Ramella M, et al.
American Journal of Translational Research, 9(12), 5485-5485 (2017)
Protocols
Enzymatic Assay of Elastase (EC 3.4.21.36), a continuous spectrophotometric rate determination.
Chromatograms
application for HPLCTudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.
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