Összes fotó(2)
Fontos dokumentumok
E0127
Elastase from porcine pancreas
Type III, lyophilized powder, Protein 55-85 %, ≥4.0 units/mg protein
Szinonimák:
Elastase from hog pancreas, Pancreatopeptidase E
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(2)
About This Item
CAS-szám:
EC-szám:
MDL-szám:
UNSPSC kód:
12352204
NACRES:
NA.54
Javasolt termékek
típus
Type III
Minőségi szint
Forma
lyophilized powder
specifikus aktivitás
≥4.0 units/mg protein
molekulatömeg
25.9 kDa
összetétel
Protein, 55-85%
oldhatóság
200 mM Tris HCl buffer, pH 8.8: soluble 1.0 mg/mL, clear
tárolási hőmérséklet
−20°C
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Alkalmazás
Elastase from porcine pancreas has been used in a study to identify a novel bone/calcium metabolism-regulating factor in porcine pancreas. Elastase from porcine pancreas has also been used in a study to investigate the effect of a specific synthetic inhibitor of neutrophil elastase (ONO-5046) on the course of acute hemorrhagic pancreatitis in dogs.
Elastase from porcine pancreas has been used in the proteolytic cleavage of UMP synthase (Uridine-5′-monophosphate synthase). It has also been used along with collagenase to disperse tissue into single cells from the thoracic media, abdominal aortic media and intima of rabbit.
Biokémiai/fiziológiai hatások
Elastase is a single polypeptide chain of 240 amino acid residues and contains four disulfide bridges. The molecular mass is approximately 25.9 kDa. The enzyme is synthesized as an inactive zymogen, proelastase, which is converted to the active form by limited proteolysis at the N-terminal by trypsin. It is a serine protease with broad specificity. It cleaves protein at the carboxyl side of small hydrophobic amino acids such as Ile, Gly, Ala, Ser, Val, and Leu. The enzyme also hydrolyzes amides and esters such as N-Benzoyl-L-alanine methyl ester. The pH optimum is found to be 8.0-8.5. It does not require any activator, but it is inhibited by diisopropyl fluorophosphate, phenylmethanesulfonyl fluoride, α2-macroglobulin, α1-antitrypsin, sulfonyl fluorides, p-dinitrophenyl diethylphosphate and high salt concentrations. Elastase is extensively used in tissue and cell dissociation procedures. It is effective in the isolation of Type II lung cells.
Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin.
Kiszerelés
Package size based on protein content
Minőség
Contains trypsin activity
Egység definíció
One unit will hydrolyze 1.0 μmole of N-succinyl-L-Ala-Ala-Ala-p-nitroanilide per min, pH 8.0 at 25 °C.
Fizikai forma
Contains sodium carbonate.
Elkészítési megjegyzés
2x crystallized and chromatographically purified
Alkalmazás
Gátló
Product No.
Leírás
Árazás
Figyelmeztetés
Danger
Figyelmeztető mondatok
Óvintézkedésre vonatkozó mondatok
Veszélyességi osztályok
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Célzott szervek
Respiratory system
Tárolási osztály kódja
11 - Combustible Solids
WGK
WGK 3
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
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Analitikai tanúsítványok (COA)
Lot/Batch Number
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R H Kelly et al.
Digestion, 22(1), 1-7 (1981-01-01)
Induction of acute hemorrhagic pancreatic necrosis by dietary means in mice produces alterations in the serum complement system. Total hemolytic complement, i.e., CH50, and native C3 levels fall during the development of pancreatitis while, at the same time, what could
J H Campbell et al.
Arteriosclerosis (Dallas, Tex.), 5(4), 318-328 (1985-07-01)
Smooth muscle cells of the rabbit aorta, when grown in vitro, express three distinguishable forms of phenotype (contractile, reversible synthetic, and irreversible synthetic). We compared the interactions of these three smooth muscle phenotypes with rabbit very low density lipoprotein (VLDL)
E E Floyd et al.
The Journal of biological chemistry, 260(16), 9443-9451 (1985-08-05)
The multifunctional protein uridine 5'-monophosphate (UMP) synthase catalyzes the final two reactions of the de novo biosynthesis of UMP in mammalian cells by the sequential action of orotate phosphoribosyltransferase (EC 2.4.2.10) and orotidine 5'-monophosphate (OMP) decarboxylase (EC 4.1.1.23). This protein
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Acute lung injury (ALI) is an early pathophysiologic change in acute respiratory distress syndrome and its management can be challenging. Omalizumab (Xolair™) is a recombinant DNA-derived, humanized antibody. OMZ-SPT is a polypeptide on the heavy chain of omalizumab monoclonal antibody.
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Taste transduction occurs in taste buds in the tongue epithelium. The Ca2+ -activated Cl- channels TMEM16A and TMEM16B play relevant physiological roles in several sensory systems. Here, we report that TMEM16A, but not TMEM16B, is expressed in the apical part
Protocols
Enzymatic Assay of Elastase (EC 3.4.21.36), a continuous spectrophotometric rate determination.
Chromatograms
application for HPLCTudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.
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