If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
93350
Trizma® base
99.7% (T), puriss. p.a.
Synonim(y):
2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol
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InChI key
LENZDBCJOHFCAS-UHFFFAOYSA-N
InChI
1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
SMILES string
NC(CO)(CO)CO
description
aminopeptidase substrate
grade
puriss. p.a.
form
crystalline
ign. residue
≤0.01% (as SO4)
pH
10.5-11.5 (25 °C, 10% in H2O)
useful pH range
7-9
Quality Level
assay
99.7% (T)
pKa (25 °C)
8.1
bp
219-220 °C/10 mmHg (lit.)
mp
167-172 °C (lit.), 168-172 °C
solubility
water: soluble (678 g/l at 20 °C)
anion traces
chloride (Cl-): ≤5 mg/kg, sulfate (SO42-): ≤5 mg/kg
cation traces
Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Na: ≤50 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Zn: ≤5 mg/kg
λ
10 % in H2O
UV absorption
λ: 230 nm Amax: ≤0.6, λ: 260 nm Amax: ≤0.05, λ: 280 nm Amax: ≤0.04, λ: 430 nm Amax: ≤0.01
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Ta pozycja | |||
|---|---|---|---|
| grade puriss. p.a. | grade - | grade - | grade - |
| assay ≥99.7% (T) | assay ≥99.9% | assay ≥99.9% (titration) | assay ≥99.9% (titration) |
| Quality Level 200 | Quality Level 100 | Quality Level 400 | Quality Level 400 |
| form crystalline | form powder | form crystalline | form crystalline powder |
| pH 10.5-11.5 (25 °C, 10% in H2O) | pH 10.5-12, 7-9 | pH 10.0-11.5 | pH 10.0-11.5 |
| solubility water: soluble (678 g/l at 20 °C) | solubility water: soluble (Water < 0.2%) | solubility water: soluble (400 g plus 600 mL of water) | solubility water: soluble (666 mg/ml, yielding a clear colorless solution) |
General description
Application
- In the preparation of TAE buffer for TAE agarose gel used to run PCR products by gel electrophoresis
- As a component of sample buffer for SDS-PAGE
- In the preparation of IEC (ion-exchange chromatography) buffer
Other Notes
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information
related product
Klasa składowania
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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Trizma Base Specification Comparison
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How can I determine the shelf life / expiration / retest date of this product?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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