T4661

Trizma^® base

≥99.9% (titration), crystalline

• Synonim(y): 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol
• Wzór liniowy: NH₂C(CH₂OH)₃
• Numer CAS: 77-86-1
• Masa cząsteczkowa: 121.14
• Beilstein: 741883
• Numer WE: 201-064-4
• Numer MDL: MFCD00004679
• Kod UNSPSC: 12352104
• Identyfikator substancji w PubChem: 24900061
• NACRES: NA.25

Właściwości

• opis: aminopeptidase substrate
• Poziom jakości: 400
• Próba: ≥99.9% (titration)
• Formularz: crystalline
• strata: ≤5% (loss on drying, 110 °C)
• kolor: white
• pH: 10.0-11.5
• przydatny zakres pH: 7-9
• pKa (25°C): 8.1
• bp: 219-220 °C/10 mmHg (lit.)
• mp: 167-172 °C (lit.)
• rozpuszczalność: water: soluble (400 g plus 600 mL of water)
• λ: (290nm < 0.05); in 5 mM Tris; DNA (40% (w/w))
• Zastosowanie: diagnostic assay manufacturing
• ciąg SMILES: NC(CO)(CO)CO
• InChI: 1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
• Klucz InChI: LENZDBCJOHFCAS-UHFFFAOYSA-N

Opis

Opis ogólny

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology.[1] It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc.[2] It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.[3]

Zastosowanie

Tris base has been used:

• As a component of lysis buffer for cell disruption[4][5]
• In the preparation of TBE solution for PAGE (polyacrylamide gel electrophoresis)[6][5]
• In studies of double stranded complexes of peptide nucleic acids (PNA) and their complementary DNA sequences, by use of anion exchange HPLC[7]
• In capillary electrochromatography and UV analysis of tocopherols and tocotrienols[8]

Opakowanie

Meets all specifications for T1503. For biotechnology, diagnostics or manufacturing use. Please inquire for commercial quantities.

Komentarz do analizy

Not pyrogen tested. Certificates of analysis are available with lot specific information.

Inne uwagi

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Informacje prawne

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: dust mask type N95 (US), Eyeshields, Gloves