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YEAST1

Sigma-Aldrich

Yeast Transformation Kit

reagents for introducing plasmid DNA into yeast

Szinonimák:

lithium acetate yeast transformation

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About This Item

UNSPSC kód:
12352200
NACRES:
NA.85

grade

for molecular biology

Minőségi szint

200

használat

 kit sufficient for >100 standard transformations

technika/technikák

transformation: suitable

kiszállítva

dry ice

tárolási hőmérséklet

−20°C

Related Categories

Általános leírás

Sigma′s Yeast Transformation Kit contains all necessary reagents and controls for efficient transformation of yeast by the lithium acetate method.

Alkalmazás

Suitable for transformation of any strain of yeast. Convenient, flexible and sensitive, positive transformants can be obtained with as little as 10 ng of DNA; the optimum efficiency is in the 0.1- 3 μg range.

Tulajdonságok és előnyök

  • Easy and ready-to-use
  • Requires as little as 10 ng of plasmid DNA
  • Flexibility for any strain of yeast
  • Sufficient for over 100 standard transformations

Komponensek

The Yeast Transformation Kit contains:
  • Transformation Buffer; 100 ml; 100 mM lithium acetate, 10 mM Tris HCl, pH 7.6, and 1 mM EDTA
  • Plate Buffer; 100 ml; 40% PEG, 100 mM lithium acetate, 10 mM Tris HCl, pH 7.5, 1 mM EDTA
  • Deoxyribonucleic acid from salmon teste, 10 mg/ml; 2 x 1 ml
  • Control Yeast Plasmid DNA pRS316 carrying the ura gene; 10 μg
  • Yeast Synthetic Drop-out Medium Supplement Without Uracil; 1 g

Elv

Transformation with a plasmid complementing the mutated gene enables the transformant to grow on medium lacking the required component. Yeast cells are made competent for transformation by incubation in a buffered lithium acetate solution. Transformation is then carried out by incubating the cells together with transforming DNA and carrier DNA in a solution containing polyethylene glycol (PEG).

Kit Components Also Available Separately

Product No.
Leírás
Biztonsági adatlap
  • D9156Deoxyribonucleic acid, single stranded from salmon testes, For hybridization 2 x 1Biztonsági adatlap
  • Y1501Yeast Synthetic Drop-out Medium Supplements, without uracil 1 gBiztonsági adatlap

kapcsolódó termék

Product No.
Leírás
Árazás

D8418

Dimethyl sulfoxide, for molecular biology

A5306

Bacteriological agar, for molecular biology

G5516

Glycerol, for molecular biology, ≥99.0%

Y1625

Yeast Extract, for use in microbial growth medium

Y0750

Yeast Synthetic Drop-out Medium Supplements, without leucine and tryptophan

Y1751

Yeast Synthetic Drop-out Medium Supplements, without histidine

Y2001

Yeast Synthetic Drop-out Medium Supplements, without histidine, leucine, tryptophan and uracil

Y1376

Yeast Synthetic Drop-out Medium Supplements, without leucine

Y0626

Yeast Nitrogen Base Without Amino Acids, Yeast classification medium used for selecting yeasts based on amino acid and carbohydrate requirements

Y1876

Yeast Synthetic Drop-out Medium Supplements, without tryptophan

Tárolási osztály kódja

10 - Combustible liquids

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

Már rendelkezik ezzel a termékkel?

Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

James R Petrie et al.
PloS one, 7(4), e35214-e35214 (2012-04-24)
Monoacylglycerol acyltransferases (MGATs) are predominantly associated with lipid absorption and resynthesis in the animal intestine where they catalyse the first step in the monoacylglycerol (MAG) pathway by acylating MAG to form diacylglycerol (DAG). Typical plant triacylglycerol (TAG) biosynthesis routes such
A two-hybrid screen identifies an unconventional role for the intermediate filament peripherin in regulating the subcellular distribution of the SNAP25-interacting protein, SIP30.
Gentil BJ
Journal of Neurochemistry, 131(5), 588-601 (2014)
S Camarero et al.
Applied and environmental microbiology, 78(5), 1370-1384 (2012-01-03)
While the Pycnoporus cinnabarinus laccase (PcL) is one of the most promising high-redox-potential enzymes for environmental biocatalysis, its practical use has to date remained limited due to the lack of directed evolution platforms with which to improve its features. Here
Miren Zumárraga et al.
Proteins, 71(1), 250-260 (2007-10-13)
The generation of diversity for directed protein evolution experiments shows an important bottleneck in the in vitro random mutagenesis protocols. Most of them are biased towards specific changes that eventually confer a predicted and conservative mutational spectrum, limiting the exploration
DMSO-enhanced whole cell yeast transformation.
J Hill et al.
Nucleic acids research, 19(20), 5791-5791 (1991-10-25)
View All Related Papers

Cikkek

Yeast Transformation Introduction

Transformation introduces exogenous DNA into cells, a fundamental genetic modification process demonstrated in Streptococcus pneumoniae.

Élesztő transzformáció Bevezetés

A transzformáció során exogén DNS-t juttatnak a sejtekbe, ami a Streptococcus pneumoniae esetében bizonyított alapvető genetikai módosítási folyamat.

Protein Expression Systems

Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.

Fehérje-expressziós rendszerek

A géntechnológia lehetővé teszi a rekombináns fehérjék kutatási célú, nagymértékű expresszióját és izolálását.

Protocols

Yeast Transformation Kit

The selection of plasmids in yeast is based on the use of auxotrophic mutant strains, which cannot grow without a specific medium component (an amino acid, purine, or pyrimidine)

Yeast Transformation Protocols

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells.

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

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