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D8418

Sigma-Aldrich

Dimethyl sulfoxide

for molecular biology

Szinonimák:

Methyl Sulfoxide, Methylsulfinylmethane, Molecular Biology Grade DMSO, DMSO

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(6)

About This Item

Lineáris képlet:
(CH3)2SO
CAS-szám:
67-68-5
Molekulatömeg:
78.13
Beilstein:
506008
EC-szám:
200-664-3
MDL-szám:
MFCD00002089
UNSPSC kód:
12191502
PubChem Substance ID:
24894214
NACRES:
NA.52

grade

for molecular biology

Minőségi szint

200

gőzsűrűség

2.7 (vs air)

gőznyomás

0.42 mmHg ( 20 °C)

termékcsalád

BioReagent

Teszt

≥99.9%

form

liquid

öngyulladási hőmérséklet

573 °F

eltarthatósági idő

Recommended retest period - 2 years

robbanási határérték

42 %, 63 °F

technika/technikák

DNA sequencing: suitable
PCR: suitable
transfection: suitable

szennyeződések

≤0.001 meq/g Titratable acid
≤0.1% water (Karl Fischer)

szín

colorless

törésmutató

n20/D 1.479 (lit.)

bp

189 °C (lit.)

mp

16-19 °C (lit.)

oldhatóság

H2O: miscible (completely)

sűrűség

1.10 g/mL (lit.)

absorption

passes test

alkalmasság

suitable for molecular biology

idegen aktivitás

DNase and RNase, none detected

tárolási hőmérséklet

room temp

SMILES string

CS(C)=O

InChI

1S/C2H6OS/c1-4(2)3/h1-2H3

Nemzetközi kémiai azonosító kulcs

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

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Általános leírás

Features and Benefits:

  • Suitable for Molecular Applications
  • Nuclease-Free DMSO
  • DNase-Free, RNase-Free, Protease-Free, Phosphatase-Free
  • Molecular Biology Grade DMSO

Dimethyl sulfoxide (DMSO) is a highly polar organic reagent that has exceptional solvent properties for organic and inorganic chemicals.
This product is designated as Molecular Biology grade and is suitable for molecular biology applications. It has been analyzed for the presence of nucleases and proteases.

Alkalmazás

DMSO (dimethyl sulfoxide) is a highly polar, aprotic organic solvent with many applications in organic chemistry and molecular biology. DMSO is routinely used in

  • polymerase chain reaction (PCR)
  • amplification of cDNA libraries
  • DNA sequencing
  • column-loading buffers for poly(A)+ RNA selection
  • buffers for the transformation of competent E. coli
  • transfection protocols.

Dimethyl sulfoxide has been used-

  • as an oligonucleotide solvent in array spotting[1]
  • as a solvent during miRNA microarray analysis and quantitative real-time PCR (qRT-PCR)
  • for cell lysis during MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay
  • for the preparation of the stock solution during MMP (mitochondrial membrane potential)cytofluorimetric assessment
  • in the storage of human and animal cell lines and bacteriophage λ as a cryoprotective agent.
To prepare a sterile filtered DMSO solution, it is recommended to use a PTFE or nylon membrane. Cellulose acetate membranes are not recommended.

Tulajdonságok és előnyök

This product is free from DNases, RNases, phosphatases and proteases.

Vigyázat

Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.

Egyéb megjegyzések

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

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Tárolási osztály kódja

10 - Combustible liquids

WGK

WGK 1

Lobbanási pont (F)

188.6 °F - closed cup

Lobbanási pont (C)

87 °C - closed cup

Egyéni védőeszköz

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

Már rendelkezik ezzel a termékkel?

Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

A Oguro-Ando et al.
Molecular psychiatry, 20(9), 1069-1078 (2014-10-15)
Rare maternally inherited duplications at 15q11-13 are observed in ~1% of individuals with an autism spectrum disorder (ASD), making it among the most common causes of ASD. 15q11-13 comprises a complex region, and as this copy number variation encompasses many
Caroline Godfrey et al.
Human molecular genetics, 24(15), 4225-4237 (2015-05-03)
Splice modulation therapy has shown great clinical promise in Duchenne muscular dystrophy, resulting in the production of dystrophin protein. Despite this, the relationship between restoring dystrophin to established dystrophic muscle and its ability to induce clinically relevant changes in muscle
C F Brayton
The Cornell veterinarian, 76(1), 61-90 (1986-01-01)
Dimethyl sulfoxide (DMSO) is a very simple compound that has stimulated much controversy in the scientific and popular literature. Fig. 1 It is an aprotic solvent. Therapeutic and toxic agents that are not soluble in water are often soluble in
Mulmudi Hemant Kumar et al.
Advanced materials (Deerfield Beach, Fla.), 26(41), 7122-7127 (2014-09-13)
Lead free perovskite solar cells based on a CsSnI3 light absorber with a spectral response from 950 nm is demonstrated. The high photocurrents noted in the system are a consequence of SnF2 addition which reduces defect concentrations and hence the
Björn Zörner et al.
Brain : a journal of neurology, 137(Pt 6), 1716-1732 (2014-04-17)
Anatomical plasticity such as fibre growth and the formation of new connections in the cortex and spinal cord is one known mechanism mediating functional recovery after damage to the central nervous system. Little is known about anatomical plasticity in the
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