Összes fotó(2)
Fontos dokumentumok
X4500
Xanthine Oxidase from bovine milk
Grade III, ammonium sulfate suspension, ≥0.8 units/mg protein
Szinonimák:
Schardinger enzyme, XnOx, hypoxanthine oxidase, XOD, Xanthine:oxygen oxidoreductase
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(2)
About This Item
CAS-szám:
Enzyme Commission szám:
1.17.3.2.
EC-szám:
MDL-szám:
UNSPSC kód:
12352204
NACRES:
NA.54
Javasolt termékek
biológiai forrás
bovine milk
Minőségi szint
típus
Grade III
Forma
ammonium sulfate suspension
specifikus aktivitás
≥0.8 units/mg protein
technika/technikák
inhibition assay: suitable
szín
faint brown to dark brown
UniProt elérési szám
idegen aktivitás
uricase ≤0.1%
tárolási hőmérséklet
2-8°C
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Általános leírás
Research area: Cell Cycle
Xanthine Oxidase (XO) belongs to the class of complex metalloflavoproteins. It is produced by oxidation of sulfhydryl residues or by proteolysis of xanthine dehydrogenase (XDH). XO is characterized with two flavin molecules (FAD), two molybdenum atoms, and eight iron atoms bound per enzymatic unit.
Xanthine Oxidase (XO) belongs to the class of complex metalloflavoproteins. It is produced by oxidation of sulfhydryl residues or by proteolysis of xanthine dehydrogenase (XDH). XO is characterized with two flavin molecules (FAD), two molybdenum atoms, and eight iron atoms bound per enzymatic unit.
Formerly E.C. 1.1.3.22
Alkalmazás
Xanthine Oxidase from bovine milk has been used:
- as a source for superoxide generation
- for the degradation of hypoxanthine
- for the detection of reactive oxygen species (ROS) in lipid model by electron spin resonance (ESR) spin trap method
- as a constituent of assay mixture in xanthine oxidase inhibition assay
Biokémiai/fiziológiai hatások
Xanthine Oxidase (XO)catalyzes the hydroxylation of hypoxanthine to xanthine and xanthine to uric acid. Xanthine oxidase activity is inhibited by folic acid. [7] and various other inhibitors including 3,4-Dihydroxy-5-nitrobenzaldehyde (DHNB), febuxostat and allopurinol. XO and xanthine dehydrogenase (XDH) plays a vital role in the last two steps in the formation of urate. Elevated levels of XO has been observed in the serum of chronic liver disease patients. Therefore, XO can be used as a biomarker for the detection of liver disease.
Xanthine oxidase activity is inhibited by folic acid.
Xanthine oxidase is a molybdenum-containing enzyme that is found in the cytosol, and may be strongly inhibited by flavonoids. It plays a vital role in the metabolism of some drugs, as well as purines and pyrimidines. It is also known to be a biological source of reactive oxygen species.
Egység definíció
One unit will convert 1.0 μmole of xanthine to uric acid per min at pH 7.5 at 25 °C. Approx. 50% of the activity is obtained with hypoxanthine as substrate.
Fizikai forma
Suspension in 2.3 M (NH4)2SO4, 10 mM sodium phosphate buffer, pH 7.8, containing 1 mM EDTA and 1 mM sodium salicylate
Elkészítési megjegyzés
Chromatographically purified
Tárolás és stabilitás
Tightly closed. Dry. Keep locked up or in an area accessible only to qualified or authorized
persons
persons
Analízis megjegyzés
Protein determined by biuret
szubsztrát
Product No.
Leírás
Árazás
Figyelmeztetés
Danger
Figyelmeztető mondatok
Óvintézkedésre vonatkozó mondatok
Veszélyességi osztályok
Resp. Sens. 1
Tárolási osztály kódja
11 - Combustible Solids
WGK
WGK 3
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Egyéni védőeszköz
Eyeshields, Gloves, type N95 (US)
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Az ügyfelek ezeket is megtekintették
C Y Ho et al.
The Journal of nutrition, 106(11), 1600-1609 (1976-11-01)
The effects of acidic and intestinal proteolytic environments on bovine milk xanthine oxidase (XO) activity were determined in order to evaluate the extent to which this enzyme was absorbed in biologically active form. The inhibition of XO by folic acid
Andrija Šmelcerović et al.
European journal of medicinal chemistry, 135, 491-516 (2017-05-10)
Xanthine oxidase (XO), a versatile metalloflavoprotein enzyme, catalyzes the oxidative hydroxylation of hypoxanthine and xanthine to uric acid in purine catabolism while simultaneously producing reactive oxygen species. Both lead to the gout-causing hyperuricemia and oxidative damage of the tissues where
Purification of immunomodulatory factors in human peripheral blood leukocytes
Montecucchi PC, et al.
Journal of Chromatography A, 512(8), 139-147 (1990)
Detection of superoxide anion radical in a stratum corneum intercellular lipid model using an electrochemical sensor
Kaneko T, et al.
Journal of Oleo Science, 60(12), 647-654 (2011)
Crystal structures of bovine milk xanthine dehydrogenase and xanthine oxidase: structure-based mechanism of conversion
Enroth C, et al.
Proceedings of the National Academy of Sciences of the USA, 97(20), 10723-10728 (2000)
Cikkek
Instructions for working with enzymes supplied as ammonium sulfate suspensions
Az ammónium-szulfát szuszpenzióként szállított enzimekkel való munkavégzésre vonatkozó utasítások
Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.
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