10110434001

Xanthine Oxidase (XOD)

from cow milk

• Szinonimák: XOD
• Enzyme Commission szám: 1.1.3.22 (BRENDA, IUBMB)
• UNSPSC kód: 12352204

Tulajdonságok

• biológiai forrás: bovine milk
• Minőségi szint: 100
• form: suspension
• specifikus aktivitás: ~1 units/mg protein (At 25 °C with xanthine as the substrate.)
• kiszerelés: pkg of 1 mL (20 U)
• gyártó/kereskedő neve: Roche
• optimális pH: 8.5-9.0
• kiszállítva: wet ice
• tárolási hőmérséklet: 2-8°C

Leírás

Általános leírás

Xanthine Oxidase (XOD) is a metal flavoprotein. It has FAD, molybdenum and iron in the ratio 2:2:8.

Xanthine:oxygen oxidoreductase

Alkalmazás

Xanthine Oxidase (XOD) has been used in the assessment of XOR-mediated NO production from NDHP and assessment of nitrite-derived NO in liver and purified XOR.

Xanthine Oxidase has been used to study tyrosine nitration.

Biokémiai/fiziológiai hatások

Xanthine Oxidase (XOD) exhibits a broad substrate specificity including aldehydes, purines and pteridines. Furthermore, this enzyme reduces oxygen to generate superoxide, hydrogen peroxide and reactive oxygen species (ROS). It also reduces nitrite to yield reactive nitrogen species (RNS), such as peroxynitrite and nitric oxide. Owing to its ability to generate RNS and ROS, XOD might play an important role as an antimicrobial agent in the neonatal gut, thereby complementing endogenous enzyme of the intestinal epithelium.

Minőség

Contaminants: <0.005% guanase, NP and uricase, each, <0.05% ADA, <0.05% alkaline phosphatase (4-nitrophenyl phosphate as the substrate)
Note: Chromatographically purified.

Szekvencia

XOD is a dimer. Each subunit contains 1 atom of molybdenum, 2 iron-sulfur centers (non-heme iron, ferredoxin-type) and 1 molecule of FAD.

Egység definíció

One unit (U) xanthine oxidase will produce 1 μmol of uric acid (E_293nm = 12.2 mmol ^-1 x L x cm^-1) from the oxidation of 1 μmol of xanthine in 1 min at 25 °C and pH8.5.

Fizikai forma

Suspension in 3.2 M ammonium sulfate solution, 10 mM EDTA, pH approximately 8

Elkészítési megjegyzés

Activator: O₂
Stabilizers: The substance is stabilized by the addition of EDTA; salicylate is not added.

Egyéb megjegyzések

For life science research only. Not for use in diagnostic procedures.

Biztonsági adatok

• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: WGK 1
• Lobbanási pont (F): does not flash
• Lobbanási pont (C): does not flash