S9697

Superoxide Dismutase bovine

recombinant, expressed in E. coli, lyophilized powder, ≥2500 units/mg protein, ≥90% (SDS-PAGE)

• Szinonimák: Superoxide Dismutase 1 bovine, cytocuprein, erythrocuprein, hemocuprein, CU/ZN-SOD, SOD, SOD1, Superoxide: superoxide oxidoreductase
• CAS-szám: 9054-89-1
• Enzyme Commission szám: 1.15.1.1 (BRENDA, IUBMB)
• EC-szám: 232-943-0
• MDL-szám: MFCD00132404
• UNSPSC kód: 12352204
• NACRES: NA.54

Tulajdonságok

• biológiai forrás: bovine
• Minőségi szint: 200
• rekombináns: expressed in E. coli
• Teszt: ≥90% (SDS-PAGE)
• form: lyophilized powder
• specifikus aktivitás: ≥2500 units/mg protein
• tárolási körülmény: (Tightly closed)
• technika/technikák: inhibition assay: suitable
• szín: white
• optimális pH: 7.8 (25 °C)
• pH-tartomány: 7.6-10.5
• pI: 4.95
• szekvencia megjegyzés: MATKAVCVLKGDGPVQGTIHFEAKGDTVVVTGSITGLTEGDHGFHVHQFGDNTQGCTSAGPHFNPLSKKHGGPKDEERHVGDLGNVTADKNGVAIVDIVDPLISLSGEYSIIGRTMVVHEKPDDLGRGGNEESTKTGNAGSRLACGVIGIAK
• NCBI elérési szám: NM_174615; NP_777040
• UniProt elérési szám: A4URH1
• tárolási hőmérséklet: −20°C

Leírás

Általános leírás

Research area: Cell Signaling

SOD from bovine erythrocytes was the first SOD to be found in mammalian tissues. There are three forms of SOD differentiated by the metal ions in the active site. These are Cu⁺²/Zn⁺², Mn⁺², and Fe⁺²SOD. In vertebrates, Cu/Zn-SOD is found in the cytoplasm, chloroplast, and may be in extracellular space, while Mn-SOD is found in the mitochondrial matrix space and peroxisome. Fe-SOD is found in the chloroplast of prokaryotes and some higher plants.

Alkalmazás

Superoxide Dismutase bovine has been used:

• to construct a calibration curve for the evaluation of superoxide dismutase (SOD) enzyme activities
• in a study to investigate where lipoproteins may affect the L-arginine-nitric oxide pathway
• in a study to investigate the mass spectral evidence for carbonate-anion-radical-induced posttranslational modification of tryptophan to kynurenine in human Cu, Zn superoxide dismutase

Biokémiai/fiziológiai hatások

Superoxide dismutase (SOD) catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. This reaction in turn activates redox-sensitive kinases and inactivates specific phosphatases to regulate redox-sensitive signaling pathway, including hypertrophy, proliferation, and migration. SOD serves as a potent antioxidant and protects the cells against the toxic effects of oxygen radicals. SOD may also suppress apoptosis by competing with nitric oxide (NO) for superoxide anion, which reacts with NO to form peroxynitrite, an inducer of apoptosis.

Egység definíció

One unit will inhibit reduction of cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25°C in a 3.0 ml reaction volume. Xanthine oxidase concentration should produce an initial ΔA550 of 0.025 ± 0.005 per min.

Elkészítési megjegyzés

Produced using animal component-free materials.

Feloldás

Reconstitute in 10 mM potassium phosphate, pH 7.4.

Analízis megjegyzés

Extinction coefficient: E^mM= 10.3 (258 nM)
SOD has no significant absorbance peak at 280 nM because of the absence of tryptophan.

Egyéb megjegyzések

Inhibitors: cyanide, OH^- (competitive), hydrogen peroxide

Biztonsági adatok

• Piktogramok: GHS08
• Figyelmeztetés: Danger
• Figyelmeztető mondatok: H334
• Óvintézkedésre vonatkozó mondatok: P261 - P284 - P501
• Veszélyességi osztályok: Resp. Sens. 1
• Tárolási osztály kódja: 10 - Combustible liquids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable