10165875001

Glucose-6-Phosphate Dehydrogenase (G6P-DH)

from Leuconostoc mesenteroides

• Szinonimák: G6P-DH, Glucose-6-Phosephate Dehydrogenase
• Enzyme Commission szám: 1.1.1.49 (BRENDA, IUBMB)
• UNSPSC kód: 12352204

Tulajdonságok

• biológiai forrás: bacterial (Leuconostoc mesenteroides)
• Minőségi szint: 100
• Forma: solution; suspension
• specifikus aktivitás: ~550 units/mg protein (At 25 °C (650 U/mg at 30 °C) with glucose-6-P and NAD as the substrates.)
• molekulatömeg: dimer 110 kDa
• kiszerelés: pkg of 1 mL (1,000 U)
• gyártó/kereskedő neve: Roche
• koncentráció: ≥0.1-1.0 % (w/w)
• technika/technikák: activity assay: suitable
• szín: white
• optimális pH: 7.0-8.5(maximal activity at 7.8)
• oldhatóság: water: miscible
• NCBI elérési szám: AAA25265
• UniProt elérési szám: P11411
• alkalmazás(ok): life science and biopharma
• idegen aktivitás: 6-PGDH <0.001%; CK <0.001%; GR <0.01%; HK <0.05%; NADH oxidase <0.02%; PGI <0.01%
• kiszállítva: wet ice
• tárolási hőmérséklet: 2-8°C

Leírás

Általános leírás

Glucose-6-Phosphate Dehydrogenase (G6PD) is a ubiquitous enzyme which acts as a catalyst in producing pentose. It also produces NADPH for various biosynthetic and detoxification reactions. Sometimes G6PD provides an alternative to main glycolytic pathway for glucose utilization. It also has its application as DNA markers on X-chromosome.1

Egyediség

Specificity: At pH 7.8, 25 °C: G6P-DH from Leuconostoc (LG6PDH) is highly specific for D-glucose-6-phosphate (K_m = 36 μM, NADP as coenzyme; 64 μM, NAD as coenzyme), but will use either NADP (K_m = 7.4 μM; relative rate 1.0) or NAD (K_m = 115 μM; relative rate 1.8) as coenzyme.
LG6P-DH does not react with fructose-6-phosphate, fructose-1,6-biphosphate, glucose-1-phosphate or ribose-1-phosphate. LG6P-DG will oxidize 2-deoxy-glucose-6-phosphate with NADP, but not with NAD as coenzyme. There is a slow reaction with D-glucose.
Heat inactivation: The ammonium sulfate suspension is not inactivated when heated to temperatures ≤ 50 °C for 10 minutes. At temperatures > 60 °C the enzyme is rapidly inactivated.

Alkalmazás

Component of cofactor recycling systems for NADPH.

It was used in enzymatic determination of C6 phosphorylation of glycogen.

Minőség

Contaminants: <0.001% CK, <0.01% GR and PGI each, <0.02% “NADH oxidase”, <0.05% HK, <0.001% 6-PGDH

Egység definíció

Unit Conversion: One unit (U) [+25 °C] ≈ 1.18 U [+30 °C] ≈ 1.45 U [+37 °C], all with NAD as coenzyme

Unit Definition: One unit (U) LG6P-DH oxidizes 1 mol of glucose-6-phosphate and reduces 1 mol of NAD in 1 minute at +25 °C and pH 7.8.

Fizikai forma

Solution of 1,000 U in 1 ml 3.2 M ammonium sulfate, pH approximately 6

Elkészítési megjegyzés

Activator: HCO^3- (≤ 0.3 M) activates slightly.

Analízis megjegyzés

Absorbance of purified enzyme: 1.15 (1 mg enzyme/ml, 280.5 nm)

Egyéb megjegyzések

For life science research only. Not for use in diagnostic procedures.

Biztonsági adatok

• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: WGK 1
• Lobbanási pont (F): does not flash
• Lobbanási pont (C): does not flash