P3397

Phosphoglucomutase from rabbit muscle

ammonium sulfate suspension, ≥100 units/mg protein

• Szinonimák: PGM, α-D-Glucose-1,6-bisphosphatase, α-D-Glucose-1-phosphate phosphotransferase
• CAS-szám: 9001-81-4
• Enzyme Commission szám: 5.4.2.2 (BRENDA, IUBMB)
• MDL-szám: MFCD00131886
• UNSPSC kód: 12352204
• NACRES: NA.54

Tulajdonságok

• biológiai forrás: rabbit muscle
• Minőségi szint: 300
• leírás: ammonium sulfate suspension, >=100 units/mg protein
• Forma: ammonium sulfate suspension
• specifikus aktivitás: ≥100 units/mg protein
• tárolási körülmény: (Tightly closed)
• technika/technikák: activity assay: suitable
• szín: white to light yellow
• idegen aktivitás: lactic dehydrogenase ≤0.5%; phosphoglucose isomerase ≤0.01%; pyruvate kinase ≤0.05%
• kiszállítva: wet ice
• tárolási hőmérséklet: 2-8°C

Leírás

Általános leírás

Research area: Cell Signaling

Phosphoglucomutase (PGM), a conserved enzyme, is abundantly found in animals, plants, and microorganisms. It is distributed in almost all tissues.[1]

Alkalmazás

Phosphoglycomutase has been used:

• to study glycogenesis and phosphoglycomutase deficiency in humans[2]
• in phosphoglucomutase assays to study metabolic regulation[3]
• for assay A to assay enzymes closely linked to glycogen metabolism in transformed BL21(DE3)C43 cells[4]
• in sucrose synthase (SuSy) andADPglucose pyrophosphorylase (AGPase) assays(3)
• in enzyme activity assay for coupling the reduction of nicotinamide adenine dinucleotide phosphate (NADP⁺)to determine glucose-1-phosphate from sucrose and inorganic phosphate (Pi)[5]

Biokémiai/fiziológiai hatások

Phosphoglucomutase(PGM) enzyme plays a vital role in glycolysis and gluconeogenesis. It is involved in glycogen and trehalose metabolism in insects. PGM participates in the development of plants and some microorganisms. It is essential for proteins, lipids, and nucleic acid metabolism and is crucial for the development of plants because glucose-6-phosphate is a crucial central metabolite. PGM catalyzes the interconversion of glucose-6-phosphate (G-6-P)and glucose-1-phosphate (G-1-P).[1]

Egység definíció

One unit will convert 1.0 μmole of α-D-glucose 1-phosphate to α-D-glucose 6-phosphate per min at pH 7.4 at 30 °C.

Fizikai forma

Crystalline suspension in 3.2 M (NH₄)₂SO₄, pH 6.0 containing 0.01% EDTA

Analízis megjegyzés

Protein determined by biuret.

Biztonsági adatok

• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: WGK 2
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)