G4134

Glucose-6-phosphate Dehydrogenase from baker′s yeast (S. cerevisiae)

Type IX, lyophilized powder, 200-400 units/mg protein (modified Warburg-Christian)

• Szinonimák: G-6-P-DH, Zwischenferment
• CAS-szám: 9001-40-5
• Enzyme Commission szám: 1.1.1.49 (BRENDA, IUBMB)
• EC-szám: 232-602-6
• MDL-szám: MFCD00081656
• UNSPSC kód: 12352204
• NACRES: NA.54

Tulajdonságok

• típus: Type IX
• Minőségi szint: 200
• form: lyophilized powder
• specifikus aktivitás: 200-400 units/mg protein (modified Warburg-Christian)
• molekulatömeg: 128 kDa
• β-NADP és β-NADPH tartalom: ≤10 mmol/mol
• alkalmazás(ok): agriculture
• kiszállítva: dry ice
• tárolási hőmérséklet: −20°C

Leírás

Általános leírás

Research area: Cell Signaling

Glucose-6-phosphate dehydrogenase (G6PD) is a key metabolic enzyme of the pentose phosphate pathway. In S. cerevisiae, it is encoded by the ZWF1 gene. G6PD exists as a tetramer in its active form.

Alkalmazás

Glucose-6-phosphate dehydrogenase is used:

• To test ketose reductase activity in developing maize endosperm.
• For recycling microassay of β-NADP and β-NADPH.
• To measure the intracellular levels of NADPH and total NADP.
• To measure the nicotinamide adenine dinucleotide (NAD) kinase kinetic assay activity.

Biokémiai/fiziológiai hatások

Glucose-6-phosphate dehydrogenase catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.

Glucose-6-phosphate dehydrogenase catalyzes the rate-limiting step in the pentose phosphate pathway. Its function involves the conversion of glucose-6-phosphate to 6-phosphogluconolacetone while generating NADPH, which is essential for the regeneration of glutathione The glutathione system utilizes nicotinamide adenine dinucleotide phosphate hydrogen (NADPH) to effectively eliminate excess hydrogen peroxide. Glucose-6-phosphate dehydrogenase (G6PD) plays an important role in regulating cell growth and survival. Their levels are higher in cells undergoing normal and neoplastic growth. Increased glucose-6-phosphate dehydrogenase activity plays a pivotal role in preventing reactive oxygen species mediated cell death. Glucose-6-phosphate dehydrogenase is over expressed in several cancers whereas its activity is reduced in hyperglycemia. A deficiency in glucose-6-phosphate dehydrogenase causes hemolysis.

Egység definíció

One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NADP at pH 7.4 at 25 °C.

Fizikai forma

Lyophilized powder essentially sulfate-free, containing approx. 20% sodium citrate

Biztonsági adatok

• Piktogramok: GHS08
• Figyelmeztetés: Danger
• Figyelmeztető mondatok: H334
• Óvintézkedésre vonatkozó mondatok: P261 - P284 - P501
• Veszélyességi osztályok: Resp. Sens. 1
• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves, type N95 (US)