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Merck
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Fontos dokumentumok

16-219

Sigma-Aldrich

Anti-c-Myc Tag Antibody

UPSTATE®, monoclonal, 4A6

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)

About This Item

UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.43
klón:
4A6, monoclonal
application:
IP
faj reaktivitás:
human
technika/technikák:
immunoprecipitation (IP): suitable
citations:
16

Terméknév

Anti-Myc Tag Antibody, clone 4A6, agarose conjugate, clone 4A6, Upstate®, from mouse

biológiai forrás

mouse

Minőségi szint

100

antitest forma

purified immunoglobulin

antitest terméktípus

primary antibodies

klón

4A6, monoclonal

faj reaktivitás

human

gyártó/kereskedő neve

Upstate®

technika/technikák

immunoprecipitation (IP): suitable

NCBI elérési szám

NM_002467.3

UniProt elérési szám

P01106

kiszállítva

wet ice

célzott transzláció utáni módosítás

unmodified

Géninformáció

human ... MYC(4609)

Általános leírás

Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.

Egyediség

Reactivity with other species has not been confirmed.

Immunogén

KLH-conjugated, synthetic peptide corresponding to amino acids 410-420 (MEQKLISEEDL) of human Myc.

Alkalmazás

Anti-Myc Tag Antibody, clone 4A6, agarose conjugate detects level of Myc Tag & has been published & validated for use in IP.
Immunoaffinity Purification:
A previous lot was used to purify Myc-tagged proteins. Elute with 100 mM tetraethyl ammonium (TEA), pH 11.5.

Tulajdonságok és előnyök

Format: Gel Immobilized

Minőség

Routinely evaluated by immunoprecipitation of Myc-tagged Akt/PKB from a COS transfected cell lysate, subsequent detection using anti-Myc Tag (05-724).

Immunoprecipitation Analysis:
4 μg of anti-Myc Tag, clone 4A6, agarose conjugate immunoprecipitated Myc-tagged Akt/PKB from 250 μg of a COS transfected cell lysate, which was then detected by immunoblot analysis, using 0.5 μg/mL of anti-Myc Tag, clone 4A6 (Catalog # 05-724).

Cél megnevezése

Varies

Fizikai forma

Purified mouse monoclonal IgG1 covalently coupled to protein G agarose beads and provided as a 50% slurry in buffer containing PBS, pH 7.4, and 0.05% sodium azide. Liquid suspension.

Tárolás és stabilitás

Stable for 1 year at 2-8ºC from date of receipt. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analízis megjegyzés

Control
Cells transfected with Myc-tagged fusion vector.

Egyéb megjegyzések

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Jogi információk

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Tárolási osztály kódja

10 - Combustible liquids

WGK

WGK 2

Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

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Dokumentumtár megtekintése

X Fu et al.
Journal of dental research, 96(12), 1451-1458 (2017-07-22)
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Dong-Lei Yang et al.
Cell discovery, 4, 55-55 (2018-10-23)
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Mutagenesis and analysis of genetic mutations in the GC-rich KISS1 receptor sequence identified in humans with reproductive disorders.
Luciana Madeira da Silva,Lauren Vandepas,Suzy D C Bianco
Journal of Visualized Experiments null
Yadong Xu et al.
The Prostate, 79(6), 657-666 (2019-02-05)
DHX15 is a member of the DEAH-box (DHX) RNA helicase family. Our previous study identified it as an AR coactivator which contributes to prostate cancer progression. We investigated DHX15 expression in castration resistant prostate cancer specimens and the influence of
Tadashi Okubo et al.
Development (Cambridge, England), 138(2), 339-348 (2010-12-24)
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