06-549
Anti-c-Myc Tag Antibody
UPSTATE®, rabbit polyclonal
Szinonimák:
Transcription factor p64, avian myelocytomatosis viral oncogene homolog, myc proto-oncogene protein, v-myc avian myelocytomatosis viral oncogene homolog, v-myc myelocytomatosis viral oncogene homolog (avian)
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(3)
About This Item
UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.43
Javasolt termékek
product name
Anti-Myc Tag Antibody, Upstate®, from rabbit
biológiai forrás
rabbit
Minőségi szint
antitest forma
purified antibody
antitest terméktípus
primary antibodies
klón
polyclonal
faj reaktivitás
human
kiszerelés
antibody small pack of 25 μg
gyártó/kereskedő neve
Upstate®
technika/technikák
immunocytochemistry: suitable
western blot: suitable
izotípus
IgG
NCBI elérési szám
UniProt elérési szám
kiszállítva
dry ice
célzott transzláció utáni módosítás
unmodified
Általános leírás
Myc proto-oncogene protein (UniProt: P01106; also known as Class E basic helix-loop-helix protein 39 (bHLHe39), Proto-oncogene c-Myc, Transcription factor p64) is encoded by the MYC (also known as BHLHE39) gene (Gene ID: 4609) in human. The Myc-tag is a short peptide sequence derived from the c-Myc protein. It is commonly used as an epitope tag in molecular biology. It is composed of 10 amino acids (EQKLISEEDL) and is fused to proteins of interest to facilitate their detection and purification. The Myc-tag functions as a marker that can be recognized by specific antibodies, allowing researchers to track the expression, localization, and interaction of tagged proteins in various experimental contexts.
Egyediség
Other species cross-reactivity not tested.
This rabbit polyclonal antibody detects human Myc and Myc-tagged recombinant proteins.
Immunogen
Epitope: a.a. 409-420
Synthetic peptide corresponding to residues 409-420 of human Myc (MEQKLISEEDLN).
Alkalmazás
Anti-Myc Tag, Cat. No. 06-549, is a rabbit polyclonal antibody that detects Myc-tagged proteins and is tested for use in Western Blotting and Immunocytochemistry.
Research Category
Epitope Tags & General Use
Epitope Tags & General Use
Research Sub Category
Epitope Tags
Epitope Tags
Tested Applications Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected Myc-tagged PP2A A subunit in NIH/3T3 cells transfected with Myc-tagged PP2A A subunit. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Minőség
Evaluated by Western Blotting in NIH/3T3 cells transfected with a Myc-tagged PP2A A subunit. Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected the Myc-tag in NIH/3T3 cells transfected with Myc-tagged PP2A A subunit.
Cél megnevezése
Predicted molecular weight for human Myc: 49 kDa Obeserved molecular weight may vary.
Fizikai forma
Format: Purified
Protein A chromatography
Purified rabbit in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl with 0.05% sodium azide. Frozen solution.
Tárolás és stabilitás
Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analízis megjegyzés
Control
Positive control for human Myc: widespread expression including A431 cell lysate, ovarian cancer cell lysate, or breast carcinoma tissue. Myc fusion protein expressed in cells.
Positive control for human Myc: widespread expression including A431 cell lysate, ovarian cancer cell lysate, or breast carcinoma tissue. Myc fusion protein expressed in cells.
Egyéb megjegyzések
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Jogi információk
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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javasolt
Tárolási osztály kódja
12 - Non Combustible Liquids
WGK
WGK 1
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
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