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Merck
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16-213

Sigma-Aldrich

Anti-Myc Tag Antibody, clone 4A6, HRP conjugate

clone 4A6, Upstate®, from mouse

Szinonimák:

Transcription factor p64, avian myelocytomatosis viral oncogene homolog, myc proto-oncogene protein, v-myc avian myelocytomatosis viral oncogene homolog, v-myc myelocytomatosis viral oncogene homolog (avian)

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.56
konjugátum:
peroxidase conjugate
application:
WB
klón:
4A6, monoclonal
faj reaktivitás:
human
citations:
14
technika/technikák:
western blot: suitable

biológiai forrás

mouse

Minőségi szint

konjugátum

peroxidase conjugate

antitest forma

purified immunoglobulin

antitest terméktípus

primary antibodies

klón

4A6, monoclonal

faj reaktivitás

human

gyártó/kereskedő neve

Upstate®

technika/technikák

western blot: suitable

NCBI elérési szám

UniProt elérési szám

kiszállítva

dry ice

célzott transzláció utáni módosítás

unmodified

Géninformáció

human ... MYC(4609)

Általános leírás

Immunogen: peptide corresponding to amino acids 410-420 (MEQKLISEEDL) of human Myc

Specificity: Recognizes and is specific for recombinant proteins containing the Myc epitope tag (EQKLISEEDL) in a variety of sequence contexts. Also recognizes human Myc.

Species Cross-reactivity: Human, others not tested

Ig Type: IgG

Host: Mouse

Molecular Weight: Varies depending upon the protein which is tagged
Myc-Tag is a short synthetic peptide sequence derived from C-terminal region of human c-myc that is used as an epitope tag. The Myc tag can be cloned either upstream or downstream of the target gene, resulting in a fusion protein that allows efficient purification, detection, and localization of target proteins. The relatively small size of this tag vs. other commonly used tags such as GST (30 kDa) makes it less likely to interfere with target protein structure or function. The myc epitope tag is widely used to detect expression of recombinant proteins in bacterial, yeast, insect, and mammalian systems.

Egyediség

Other species cross-reactivity not tested.
Recognizes and is specific for recombinant proteins containing the Myc epitope tag (EQKLISEEDL) in a variety of sequence contexts. Also recognizes human Myc.

Immunogén

Epitope: C-terminal
KLH-conjugated, synthetic peptide corresponding to amino acids 410-420 (MEQKLISEEDL) of human Myc. Clone 4A6.

Alkalmazás

Anti-Myc Tag Antibody, clone 4A6, HRP conjugate detects level of Myc Tag & has been published & validated for use in WB.
Research Category
Epitope Tags & General Use
Research Sub Category
Epitope Tags

Minőség

Evaluated by Western Blot on Myc-PP2A A subunit transfected NIH/3T3 lysates.

Western Blot Analysis:
1:500 dilution of this antibody detected myc-PP2A on 10 µg of Myc-PP2A A subunit transfected NIH/3T3 lysates.

Cél megnevezése

Varies depending upon the protein which is tagged.

Fizikai forma

Protein G Purified
Purified mouse monoclonal IgG1 conjugated to horseradish peroxidase (HRP) in buffer containing: 50% storage buffer (PBS, 0.05% Kathon®, 0.2 mg/mL BSA) and 50% glycerol.

Tárolás és stabilitás

Stable for 1 year at -20ºC from date of receipt.

Analízis megjegyzés

Control
Positive control for human Myc: Widespread expression including A431 cell lysate, ovarian cancer cell lysate, or breast carcinoma tissue. Myc fusion protein expressed in cells.

Egyéb megjegyzések

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Jogi információk

Kathon is a registered trademark of Rohm and Haas Co.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Piktogramok

Exclamation markEnvironment

Figyelmeztetés

Warning

Figyelmeztető mondatok

Óvintézkedésre vonatkozó mondatok

Veszélyességi osztályok

Aquatic Chronic 2 - Skin Sens. 1

Tárolási osztály kódja

10 - Combustible liquids

WGK

WGK 3

Lobbanási pont (F)

390.2 °F - closed cup - Information refers to the main ingredient.

Lobbanási pont (C)

199 °C - closed cup - Information refers to the main ingredient.


Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

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Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

Catalytic assembly of the mitotic checkpoint inhibitor BubR1-Cdc20 by a Mad2-induced functional switch in Cdc20.
Han, JS; Holland, AJ; Fachinetti, D; Kulukian, A; Cetin, B; Cleveland, DW
Molecular Cell null
A novel transgenic mouse model of growth plate dysplasia reveals that decreased chondrocyte proliferation due to chronic ER stress is a key factor in reduced bone growth.
Gualeni, B; Rajpar, MH; Kellogg, A; Bell, PA; Arvan, P; Boot-Handford, RP; Briggs, MD
Disease models & mechanisms null
Hee Jun Cho et al.
Journal of microbiology and biotechnology, 30(10), 1488-1494 (2020-09-03)
Rho guanine nucleotide dissociation inhibitor 1 (RhoGDI1) plays important roles in numerous cellular processes, including cell motility, adhesion, and proliferation, by regulating the activity of Rho GTPases. Its expression is altered in various human cancers and is associated with malignant
Junying Wang et al.
Nature communications, 15(1), 3481-3481 (2024-04-26)
Viral myocarditis, an inflammatory disease of the myocardium, is a significant cause of sudden death in children and young adults. The current coronavirus disease 19 pandemic emphasizes the need to understand the pathogenesis mechanisms and potential treatment strategies for viral
Erpeng Wu et al.
Cell research (2023-08-15)
Ultra-stable fibrous structure is a hallmark of amyloids. In contrast to canonical disease-related amyloids, emerging research indicates that a significant number of cellular amyloids, termed 'functional amyloids', contribute to signal transduction as temporal signaling hubs in humans. However, it is

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