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M2154

Sigma-Aldrich

Medium 199

With Earle′s salts and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Szinonimák:

M199 Medium, TCM 199

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)

About This Item

UNSPSC kód:
12352207
NACRES:
NA.75

Terméknév

Medium 199, With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Minőségi szint

400

sterilitás

sterile-filtered

Forma

liquid

technika/technikák

cell culture | mammalian: suitable

szennyeződések

endotoxin, tested

pH

7.0-7.6 (25 °C, 209 g/L)

alkatrészek

sodium pyruvate: no
L-glutamine: no
NaHCO3: yes
HEPES: no
phenol red: yes
Earle’s salts (5% CO2): yes

kiszállítva

ambient

tárolási hőmérséklet

2-8°C

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Általános leírás

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Alkalmazás

Medium 199 has been used:
  • to culture rumen epithelial cells from yearling Speckle Park beef heifers
  • as a component of the maturation medium for the incubation of Cumulus oocyte complexes (COCs) from bovine ovaries
  • as a component of the Dulbecco′s modified Eagle medium (DMEM) mixture to maintain rat neonatal cardiac myocytes (NCM)

Feloldás

Supplement with 0.1 g/L L-glutamine.

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Leírás
Árazás

kiegészítés

Product No.
Leírás
Árazás

Tárolási osztály kódja

12 - Non Combustible Liquids

WGK

WGK 1

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Válasszon a legfrissebb verziók közül:

Analitikai tanúsítványok (COA)

Lot/Batch Number
RNBL2658
RNBK5880
RNBJ2362
RNBH5039
RNBH0059

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Dokumentumtár megtekintése

E Held-Hoelker et al.
Theriogenology, 96, 145-152 (2017-05-24)
Lipid accumulation is associated with reduced embryonic quality, causing limited survival after cryopreservation. Therefore, in the present study we aimed to reveal the effects of supplementation of a lipid reducing agent, l-carnitine and the removal of fatty acids during in vitro
C Kent-Dennis et al.
Journal of dairy science, 104(1), 1002-1017 (2020-11-03)
Inflammation of ruminal epithelium may occur during ruminal acidosis as a result of translocation and interaction of ruminal epithelial cells (REC) with molecules such as lipopolysaccharide (LPS). Such inflammation has been reported to alter cellular processes such as nutrient absorption
Michelle M Halstead et al.
Nature communications, 11(1), 4654-4654 (2020-09-19)
The shift from maternal to embryonic control is a critical developmental milestone in preimplantation development. Widespread transcriptomic and epigenetic remodeling facilitate this transition from terminally differentiated gametes to totipotent blastomeres, but the identity of transcription factors (TF) and genomic elements
Alfred Nguyen et al.
Lab on a chip, 21(10), 1929-1947 (2021-05-20)
Active microrheology is one of the main methods to determine the mechanical properties of cells and tissue, and the modelling of these viscoelastic properties is under heavy debate with many competing approaches. Most experimental methods of active microrheology such as
Nigel J Brand et al.
Methods in molecular biology (Clifton, N.J.), 633, 113-124 (2010-03-06)
We describe a method of isolating and maintaining primary cultures of mouse neonatal cardiac myocytes (NCM). This is derived from the well-established procedure for making NCM cultures from rat neonates by sequential digestion of rat ventricular myocardial pieces using a
View All Related Papers

Cikkek

Medium 199 - M199 Formulations

Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

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