D5546

DMEM - low glucose

With sodium bicarbonate and sodium pyruvate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

• Szinonimák: DME, Dulbecco′s Modified Eagle′s Medium - high glucose, DMEM
• MDL-szám: MFCD00217342
• UNSPSC kód: 12352207
• NACRES: NA.75

Tulajdonságok

• product name: Dulbecco′s Modified Eagle′s Medium - low glucose, With 1000 mg/L glucose, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
• Minőségi szint: 400
• sterilitás: sterile-filtered
• form: liquid
• technika/technikák: cell culture | mammalian: suitable
• szennyeződések: endotoxin, tested
• alkatrészek: NaHCO₃: yes; phenol red: yes; glucose: low; HEPES: no; sodium pyruvate: yes; L-glutamine: no
• kiszállítva: ambient
• tárolási hőmérséklet: 2-8°C

Leírás

Általános leírás

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains a higher concentration of amino acids, vitamins, and additional supplementary components. The original DMEM formula, first reported for culturing embryonic mouse cells, contained 1,000 mg/L of glucose. An alteration with 4,500 mg/L glucose is optimal in cultivating certain cell types. This modification has been shown to improve the stability of the medium. 

Alkalmazás

Dulbecco′s Modified Eagle′s Medium - low glucose has been used to culture:

• human osteosarcoma cells and HeLa cells
• primary human mesenchymal stromal cells (hBMSCs)
• human embryonic kidney cell lines

Feloldás

Supplement with 0.584 g/L glutamine.

Biztonsági adatok

• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable