Ugrás a tartalomra
Merck

F2442

Sigma-Aldrich

Fetal Bovine Serum

USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma

Szinonimák:

Fetal Bovine Sera, Fetal Calf Serum, FBS, FCS, sera, serum

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

MDL-szám:
UNSPSC kód:
12352207
eCl@ss:
32160409
NACRES:
NA.71

biológiai forrás

bovine fetus

Minőségi szint

sterilitás

sterile-filtered

termékcsalád

FBS Classic

összetétel

Bovine IgG, ≤1 mg/mL
Hemoglobin, ≤20 mg/dL

eredet

USA origin

technika/technikák

cell culture | hybridoma: suitable
cell culture | mammalian: suitable

szennyeződések

≤10 EU/mL endotoxin

kiszállítva

dry ice

tárolási hőmérséklet

−20°C

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Általános leírás

Fetal Bovine Serum (FBS) is a complex mixture with low and high molecular weight biomolecules exhibiting optimal growth-enhancing and growth-suppressing functions. FBS contains molecules, such as growth factors, proteins, vitamins, trace elements, hormones etc. These molecules are important for the growth and maintenance of cells. It is a common supplement for cell culture media. FBS is derived from the calf blood. It is devoid of fibrin and clotting factors. Heat inactivation of FBS blocks the complement system. FBS derived from clotted blood is the most widely used undefined supplement in eukaryotic, especially mammalian, cell culture. It is typically used with the classical media that were developed in the 1950s and 1960s. These include many popular media such as Minimum Essential Medium (MEM), Dulbecco′s Modified Eagles Media (DME, DMEM); Iscove′s Modified Eagles Medium (IMDM), M199 and Roswell Park Memorial Institute medium, RPMI-1640.

Alkalmazás

Fetal Bovine Serum has also been used as a component of:
  • perivascular-resident macrophage-like melanocytes (PVM/M) growth medium
  • synthetic oviduct fluid (SOF)
  • Eagle′s minimum essential medium (EMEM)
  • neurobasal medium
  • Hyclone McCoys 5 A medium
  • Hanks medium

Biokémiai/fiziológiai hatások

FBS remains a popular media supplement because it provides a wide array of functions in cell culture. FBS delivers nutrients, growth and attachment factors and protects cells from oxidative damage and apoptosis by mechanisms that are difficult to reproduce in serum-free media (SFM) systems.

Analízis megjegyzés

  • Endotoxin and hemoglobin tested
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions

Egyéb megjegyzések

Tested for the presence of bacteria, virus, mycoplasma, endotoxin and for hemoglobin content. Triple filtered with 0.1 micron membrane under aseptic conditions.

Tárolási osztály kódja

10 - Combustible liquids

WGK

WGK 3

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Egyéni védőeszköz

Eyeshields, Gloves


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Analitikai tanúsítványok (COA)

Lot/Batch Number

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Dokumentumtár megtekintése

Az ügyfelek ezeket is megtekintették

Axenic Procyclic Culture of L. tropica and L. donovani in Culture of FBS-free Medium
Mosa FM, et al.
Ife Journal of Science, 4B, 3088-3093 (2015)
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Grigoryev SA et al.
The Journal of Biological Chemistry, 274, 5626-5626 (1999)
Inhibiting tyrosine phosphorylation of protein kinase Cd (PKCd) protects the salivary gland from radiation damage.
Wie SM et al.
The Journal of Biological Chemistry, 289, 10900-10900 (2014)
Cunzhi Yu et al.
Frontiers in pharmacology, 8, 832-832 (2017-12-07)
Low solubility, tissue accumulation, and toxicity are chief obstacles to developing triptolide derivatives, so a better understanding of the pharmacokinetics and toxicity of triptolide derivatives will help with these limitations. To address this, we studied pharmacokinetics and toxicity of (5R)-5-hydroxytriptolide
Optimization of chemically defined cell culture media--replacing fetal bovine serum in mammalian in vitro methods.
van der Valk J et al.
Toxicology in vitro, 24, 1053-1053 (2010)

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