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Merck

M0650

Sigma-Aldrich

Medium 199

With Earle′s salts, without ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture, 10X

Szinonimák:

Cell growth medium, M199 Medium, TCM 199

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

UNSPSC kód:
12352207
NACRES:
NA.75

product name

Medium 199, 10 ×, With Earle′s salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Minőségi szint

sterilitás

sterile-filtered

form

liquid

koncentráció

10 ×

technika/technikák

cell culture | mammalian: suitable

szennyeződések

endotoxin, tested

alkatrészek

NaHCO3: no
phenol red: yes
sodium pyruvate: no
HEPES: no
Earle’s salts (5% CO2): yes
L-glutamine: no

kiszállítva

ambient

tárolási hőmérséklet

2-8°C

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Általános leírás

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Alkalmazás

Medium 199 has been used:

  • to culture human mesothelial cells
  • as a component of the holding medium for the collection of embryos from pluriparous Boer goat
  • in the preparation of spheroid-embedded collagen matrices

Feloldás

Supplement with 0.1 g/L L-glutamine, 2.2 g/L sodium bicarbonate at 1×.

Tárolási osztály kódja

12 - Non Combustible Liquids

WGK

WGK 2

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable


Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

Már rendelkezik ezzel a termékkel?

Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

Technical Note: Transfer of caprine blastocysts vitrified by the open pulled straw (OPS) or the solid surface procedure and warmed in sucrose-free medium
Garza D, et al.
Small Ruminant Research, 165, 111-114 (2018)
Elena García-Gareta et al.
BioResearch open access, 2(6), 412-420 (2014-01-01)
The clinical demand for effective dermal substitutes continues as current commercially available products present limitations. However, there are no definitive in vitro methods to predict in vivo outcomes such as integration, cellularization and contraction, which may help the development of
Ming Yu et al.
Nature metabolism, 2(10), 1013-1020 (2020-09-09)
The intrahepatic milieu is inhospitable to intraportal islet allografts1-3, limiting their applicability for the treatment of type 1 diabetes. Although the subcutaneous space represents an alternate, safe and easily accessible site for pancreatic islet transplantation, lack of neovascularization and the resulting
Klaus Kratochwill et al.
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, 27(3), 937-946 (2011-08-23)
Exposure of mesothelial cells to peritoneal dialysis fluids (PDF) results in cytoprotective cellular stress responses (CSR) that counteract PDF-induced damage. In this study, we tested the hypothesis that the CSR may be inadequate in relevant models of peritoneal dialysis (PD)
Woo-Jin Shin et al.
mBio, 9(6) (2018-11-30)
As oral poliovirus vaccine (OPV) causes vaccine-associated paralytic poliomyelitis, the polio endgame strategy introduced by the Global Polio Eradication Initiative calls for a phased withdrawal of OPV and an introduction of inactivated poliovirus vaccine (IPV). The introduction of IPV creates

Cikkek

Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

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