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M3769

Sigma-Aldrich

Medium 199

Modified, with Earle′s salts, without L-glutamine, sodium bicarbonate, and phenol red, powder, suitable for cell culture

Szinonimák:

M199 Medium, TCM 199

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About This Item

UNSPSC kód:
12352207
NACRES:
NA.71

Minőségi szint

500

Forma

powder

technika/technikák

cell culture | mammalian: suitable

alkatrészek

L-glutamine: no
phenol red: no
HEPES: no
sodium pyruvate: no
Earle’s salts (5% CO2): yes
NaHCO3: no

kiszállítva

ambient

tárolási hőmérséklet

2-8°C

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Általános leírás

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Alkalmazás

Medium 199 has been used:
  • to culture the primary human umbilical vein endothelial cells (HUVECs) for use in cell viability assay
  • to flush the uterine horn of gilts to recover day 12 conceptuses from uteri
  • to culture the peritoneal cells and immortalized human mesothelial cells (MeT-5A and ATCC-CRL-9444)

Mennyiség

Formulated to contain 9.4 grams of powder per liter of medium.

Feloldás

Supplement with 0.1 g/L L-glutamine, 2.2 g/L sodium bicarbonate.

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Leírás
Árazás

kiegészítés

Product No.
Leírás
Árazás

Tárolási osztály kódja

13 - Non Combustible Solids

WGK

WGK 1

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Válasszon a legfrissebb verziók közül:

Analitikai tanúsítványok (COA)

Lot/Batch Number
0000322679
0000321888
0000321588
0000321349
0000321744

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COA keresése

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Dokumentumtár megtekintése

Luciana de Lima Ferreira et al.
PLoS neglected tropical diseases, 14(12), e0009015-e0009015 (2020-12-29)
Trypanosoma rangeli is a non-pathogenic protozoan parasite that infects mammals, including humans, in Chagas disease-endemic areas of South and Central America. The parasite is transmitted to a mammalian host when an infected triatomine injects metacyclic trypomastigotes into the host's skin
Diane G Edmondson et al.
mBio, 12(1) (2021-02-25)
The bacterium that causes syphilis, Treponema pallidum subsp. pallidum, has now been cultured in vitro continuously for periods exceeding 3 years using a system consisting of coculture with Sf1Ep rabbit epithelial cells in TpCM-2 medium and a low-oxygen environment. In
Klaus Kratochwill et al.
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, 27(3), 937-946 (2011-08-23)
Exposure of mesothelial cells to peritoneal dialysis fluids (PDF) results in cytoprotective cellular stress responses (CSR) that counteract PDF-induced damage. In this study, we tested the hypothesis that the CSR may be inadequate in relevant models of peritoneal dialysis (PD)
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Colloids and surfaces. B, Biointerfaces, 208, 112136-112136 (2021-10-11)
Nitric oxide (NO) is a potential therapeutic agent for various diseases. However, it is challenging to deliver this unstable, free-radical gaseous molecule in the body. Various nanoparticle-based drug delivery systems have been investigated as promising NO carriers without detailed characterization
J L N Barratt et al.
Parasitology, 137(13), 1867-1878 (2010-07-09)
Dientamoeba fragilis is a pathogen of the human gastrointestinal tract that is a common cause of diarrhoea. A paucity of knowledge on the in vitro cultivation and cryopreservation of Dientamoeba has meant that few studies have been conducted to investigate
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Cikkek

Medium 199 - M199 Formulations

Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.

Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

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