L4919

Lysozyme from chicken egg white

BioUltra, lyophilized powder, ≥98% (SDS-PAGE), ≥40,000 units/mg protein

• Szinonimák: Mucopeptide N-acetylmuramoylhydrolase, Muramidase
• CAS-szám: 12650-88-3
• Enzyme Commission szám: 3.2.1.17 (BRENDA, IUBMB)
• EC-szám: 235-747-3
• MDL-szám: MFCD00131557
• UNSPSC kód: 12352204
• NACRES: NA.54

Tulajdonságok

• biológiai forrás: chicken egg white
• Minőségi szint: 200
• grade: for molecular biology
• termékcsalád: BioUltra
• Teszt: ≥98% (SDS-PAGE)
• form: lyophilized powder
• specifikus aktivitás: ≥40,000 units/mg protein
• molekulatömeg: single-chain 14.3 kDa
• összetétel: Protein, ≥90%
• technika/technikák: cell based assay: suitable
• alkalmasság: suitable for cell lysis
• alkalmazás(ok): cell analysis
• tárolási hőmérséklet: −20°C

Leírás

Általános leírás

Lysozyme is abundantly found in animal and plant kingdoms. It is a natural food preservative and also found to be present in specific bacterial cell walls. Lysozyme is usually present in tears, milk, urine and saliva.

Alkalmazás

Lysozyme from chicken egg white has been used:

• in dielectric spectroscopy studies of dynamics of protein
• as a control to measure the human lysozyme activity
• as a supplement in soaking solution to treat lenses

Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.

Biokémiai/fiziológiai hatások

Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.

The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).

Lysozymes participate in the defense mechanism. It has the ability to stimulate catalysis by bringing steric stress in the substrates.

Tulajdonságok és előnyök

• Highly purified by repeated crystallization and dialysis
• Each lot is use-tested for isolation of plasmid DNA from E. coli

Egység definíció

One unit will lyse 0.6 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 6.2 at 25 °C.

Elkészítési megjegyzés

3× crystallized

Biztonsági adatok

• Piktogramok: GHS08
• Figyelmeztetés: Danger
• Figyelmeztető mondatok: H334
• Óvintézkedésre vonatkozó mondatok: P261 - P284 - P501
• Veszélyességi osztályok: Resp. Sens. 1
• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves, type N95 (US)